Regulation Of TLR4 Signaling In Macrophages By Zinc Finger Protein Zbtb20

In this study we investigated the regulation of TLR4 signaling in macrophages by zinc finger protein zbtb20. We found that silencing of zbtb20 could inhibit LPS-induced production of proinflammatory cytokines and interferon-beta in macrophages and accordingly suppress activation of MAPK (ERK, JNK and p38) pathway. The similar phenomenon was obserbed in LPS-stimualted macrophages derived from zbtb20-deficient mice. In contrast, zbtb20 overexpression promoted TLR4-triggered cytokine production and MAPK activation. Therefore, we demonstrate that zbtb20 may function as a positive regulatory molecule in TLR4 signaling.