| To investigate the correlation and clinical significance of the expressions and effects of Thl cytokines on survival time of patients with gastric cancer after radical resection.We selected specimens from 58 patients who underwent RO resections with extended lymph nodes dissection(D2) from June 2006 through January 2008 at Zhongshan Hospital (Shanghai, China). The date of last follow-up was February 2010. The expressions of Thl cytokines mRNA was detected in tumor tissues from 58 patients with gastric cancer by real-time polymerase chain reaction (RT-PCR) method. Prognostic effects of Thl cytokines were evaluated by Cox regression and Kaplan-Meier analysis using median values as cutoff.At last follow-up,13 patients (22.4%) studied had died, all of whom died from the recurrence of gastric cancer. The median duration of follow-up were 30.5 months (range,1.0-43.0). The overall survival (OS) rates were 84.5% for 1 year,80.6% for 2 years, respectively, for the whole study. The mRNA expressions of GNLY, GZMB and IFNG are associated with tumor emboli. The mRNA expressions of TRAV10, IRF1, TBX21, CD3Z, GZMB, GATA3 and IFNG are not associated with OS. In the tumor sites, the lower mRNA expression of GNLY is associated with worse OS (Urivariate analysis HR 0.23, p=0.022), however, it is not an independent predictor. At the same time, the combination of mRNA expressions of eight cytokines is an independent factor for OS (Urivariate analysis HR 0.23,p=0.001; Mutlivariate analysis HR 0.17,p=0.05).1-year and 2-year OS rates were 98%,89.8% for the group with high mRNA expression of eight cytokines, compared with 66.7%,55.6% for the group with low mRNA expression (Logrank-testp<0.001).1. The mRNA expression of GNLY is associated with OS, but it is not an independent factor.2. The combination of mRNA expressions of the eight cytokines is an independent factor for OS.3. The mRNA expression of GNLY, GZMB and IFNG is separately negatively correlated with tumor emboli.
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