Study On Feature Of Expression Of Cytochrone C Oxidase Subunits â…  And â…£ In Hippocampus Of Epileptic Rats Induced By Penicillin And On The Mechanism Of Intervention Therapy With Metal Electrode

Objective:Epilepsy is a syndrome caused by synchronization discharge of nerve network in central nervous system. The electric current origin from the flowing of electric charge, which drived by the difference of potential. Energy provided by biological oxidation is the driving force for the forming of difference of potential. To demonstrate the dynamic change of epileptic power in neuron during seizure onsets, we measured the electric potential of epileptogenic focus and the expression of Cytochrone C Oxidase SubunitsⅠ(COXⅠ)andⅣ(COXⅣ)in hippocampus of epileptic model induced by penicillin were observed.To explore the mechanism of metal electrode's anti-epilepsy function, the effect of metal electrode on electric potential of epileptogenic focus and the expression of COXⅠand COXⅣin hippocampus of epileptic model induced by penicillin were observed.Methods:50 Sprague-Dauley rats were divided randomly into 4 groups(10 subgroups), including saline control group(A),1 hour after seizure onset group (B1),3 hour after seizure onset group (B3),24 hour after seizure onset group (B24),1 hour after seizure onset with interfering of metal electrode group (C1),3 hour after seizure onset with interfering of metal electrode group (C3),24 hour after seizure onset with interfering of metal electrode group (C24),1 hour after seizure onset with interfering of insulation electrode group (D1),3 hour after seizure onset with interfering of insulation electrode group (D3), 24 hour after seizure onset with interfering of insulation electrode group (D24).The animals of the 10 subgroups were evaluated in behavior and the latent period (the time from penicillin injection to epilepsy onset which Racine grade was 2 or above) were recorded. The EEG were recorded and the electric potential of epileptogenic focus were measured too.The expression of COXⅠand COX IVin rat hippocampus were detected by immunohistochemistry.Results:1. Behavioral changesThe latent period of seizure:the latent period in groups B was 12.52±4.18 minuts, D was 12.08±3.90 minuts and latent period in groups C was 16.08±5.4 minuts, which was longer than that in group B and group D(p<0.05).Intensity of seizures:in groups B and D, at 1h after seizure onset, the intensity of seizures were at Racine 4 in the majority of rats, at 3h after seizure onset, the intensity of seizures were at Racine5 in the majority of rats. in groups C, at 1h after seizure onset, the intensity of seizures were at Racine 3-4 in the majority of rats, at 3h after seizure onset, the intensity of seizures were at Racine 4-5 in the majority of rat2. The changes of EEG Main waves in EEG of group A wereα,βwaves, occasionallyθwaves, no epileptic waves were seen.In all groups, before seizure onsets most of the epileptic waves were paroxysmal sharp waves and spike waves.At 3h after seizure onsets, persistant sharp waves, spike waves, sharp and slow wave complexes were showed in EEG.low amplitude epileptic waves could still be found on EEG, although there were no behavioral epileptic seizures.In group C(C1, C3, C24), the frequenies and amplitudes of epileptic waves were decreased.3. The changes of electric potential of epoleptogenci focusThe electric potential of epoleptogenci focus significantly higher in group B,group C,group D, when compared with group A.In group A, the electric potential of epoleptogenci focus have no significantly difference at every time point.Group B3,C3,A3 showed significantly higher electric potential than group B1 C1 D1 respectively, the electric potential significantly lower in group B24,C24,D24 when compared with group B3,C3,D3 respectively.At the same time point, the electric potential significantly lower in group C when compared with group B and D. And there were no significantly difference of electric potential between group B and D.4. Comparions of the expression of COXⅠand COXⅤ4.1 Expression of COXⅠin hippocampusThe expression of COXⅠsignificantly increased in all the groups at 1h after seizure onsets when compared with the control group. COXⅠshowed significantly increased expression in all the groups at 3h (B3,C3,D3) when compared with the groups at 1h after seizure onsets(B1,C1,D1). The expression of COXⅠsignificantly decreased in all the groups at 24h after seizure onsets (B24,C24,D24), which were similar to that in control group.In the same time point, the expression of COX I have no significant difference among group B,C and D.4.2 Expression of COXⅣin hippocampusThe expression of COXⅣsignificantly increased in all the groups at 1h after seizure onsets when compared with the control group. COXⅣshowed significantly increased expression in all the groups at 3h (B3,C3,D3) when compared with the groups at 1h after seizure onsets(B1,C1,D1). The expression of COXⅣsignificantly decreased in all the groups at 24h after seizure onsets (B24,C24,D24), which were similar to that in control group.In the same time point, the expression of COXIV have no significant difference among group B,C and D.Conclusion:1. The electric potential of epileptogenic focus and the expression of COXⅠand COXⅣin hippocampus all changed regularly, which manifested that there were dynamic change of epileptic power in neuron network during seizure onsets.2. The latent period prolonged and the epileptogenic focus'electric potential decreased when interfered with the metal electrode, which illustrated that metal electrode was involved in the shunting of epileptic power during process of epilepticgensis.