A Novel Extraction Of Coptis Chinentsis Complex Anti-metastasis Of Nasopharyngeal Carcinoma

Objective:Nasopharyngeal carcinoma (NPC) has a high metastasis, high recurrence and low differentiate. These set a difficult question for NPC therapy. To search for a novel anti- metastasis drug for NPC which has characteristics including anticancer effect, low toxicity, high purity. We use HPLC to extract a component of Qi-Boosting Toxin-Resolving Granule, which has been to treat NPC patients with radiotherapy and has a well therapeutic effect. The extracts were confirmed their anti-metastasis effect. It will provide a new strategy and method to develop new drugs for anti-NPC. At the same time, we provide evidences for developing a new drug in vitro.Method:1. A complex, Qi-Boosting Toxin-Resolving Granule containing Coptis chinensis, which generally was used to treat NPC patients and has a well clinic effect, was separated using HPLC.2. The extracts were used to treated NPC cell (CNE1) and get an effective component. This component structure was determined by Nuclear Magnetic Resonance (NMR), Liquid Chromatography-Mass Spectrometry (LC-MS).3. The abilities of proliferation of the component on CNE1 cells were analyzed by MTT assay. To observe the toxicity of the component, LDH was measured in media of cells with drug treatment. We used FITC-phalloidin to analyze the drug affect on cytoskeleton. The abilities of migration were observed by scratch assay and transwell assay.4. SPSS 15.0 was used to analyze the data. Results:1. MTT assay data showed that Component 7 was an effective component, could inhibit the proliferation of CNE1 cells at the concentration greater than 20μmol/L (P<0.05), and no effect less than 20μmol/l.0-20μmol/l is the non-cytotoxic concentration (NCC) of the component.2. The chemical formula of Component 7 is C21H22O9, and the molecular weight is 418.3. LDH results showed that the concentration of LDH in the culture medium was lower after Component 7 treated CNE1 cells compared with berberine.4. FITC-phalloidin staining results showed that Component 7 could inhibit the growth of cytoskeleton and filopodia of CNE1.5. Scratch assay and transwell results showed that Component 7 could dramatically inhibit the migration of CNE1.Conclusions:1. We successfully extracted and separated components of Qi-Boosting Toxin-Resolving Granule by use HPLC and got a novel component for NPC therapy. This provides a novel strategy NPC therapy.2. The effect component could inhibit the morphology, cytoskeleton, and migration ability of CNE1 cells at non-cytotoxic concentration.3. Clinic anti-metastasis of Qi-Boosting Toxin-Resolving Granule may be determined by this effective component.