Edaravone Pretreatment On Rats' Cerebral Ischemia Reperfusion Apoptosis And The Effects On Bcl-2 And Bax Gene Expression

Objective:To investigate the effects of EDA preconditioning on apoptosis and expression of Bcl-2 and Bax in rats following focal cerebral ischemia and reperfusion. To explore protective effects of EDA as anti-apoptotic drugs on the neurons and the optimal dosage.Methods:Established the rat MCA cerebral ischemia/ reperfusion(CI/RP)model with suture occlusion technique according to modified Zea Longa method and then the rats were randomly devided into two groups:one contrast group and one therapy group with EDA(3mg/kg).After consciousness the neurologic impairment evaluation score were recorded. The rats were then killed at the sixth day and some of the brains were removed to measure the infarct volume,the others were detected apoptosis by TUNEL technology and protein Bcl-2 and Bax/Bad levels by western-blot technology.Results:Neurologic impairment in various degree appeared in the contrast group rat after CI/RP.The infarct brain area, where showed white colour because not stained by TTC,can be distinguished clearly to the normal brain where showed red colour. TUNEL positive cell which call represent apoptosic neurous mainly appeared in the penumbra.Protein Bcl-2 increased and Bax/Bad decreased by western-blot.Conclusion:1.The effect of EDA pharmacological preconditioning Can reduce infarct volume; 2.The effect of EDA pharmacological preconditioning can decrease neurologic impairment;3.The effect of EDA pharmacological preconditioning Can inhibit the neuron apoptosis of isehemie area via enhanced protein Bcl-2 expression as well as reduced protein Bax and Bad expression.