Experimental Studies On Inhibition Of CVS After SAH By Panax Notogi-nosid (PNS)

Objective:To study the PNS'S effects of CVS after SAH in rats and its impact on the expression of TNF-αchanges in the cerebrovascular wall, in order to verify whether PNS could effectively prevent or mitigate the brain damage caused by CVS after SAH by inhibiting the expression of TNF-αin the cerebrovascular in rats。Methods:Randomly divide the SD rats into ten groups: sham-operated group (3-days,7-days), saline group (3-days,7-days), SAH group(3-days,7-days), SAH with PNS intervention group(3-days,7-days) and PNS pretreatment before SAH group (3-days,7-days) Surgeries were operated for modeling. Then we scored on animal neurological behaviour at 3rd day,7th day after modeling, removed brain pontine basilar artery to observe the sections of blood vessels spasming and morphology changes of cerebrovascular walls in biopsy, and used immunohistochemical method to identify the expression changes of vascular wall inflammation indicators TNF-α. The data was processed by Statistics finally。Results:There was significant statistical difference(P<0.05)between PNS treatment group/PNS pretreatment group and SAH group at the time of 3rd day and 7th day on animals neurobehavior score, and the same significant statistical difference (P<0.05) on basilar artery wall thickness。 The SAH group showed the strongest TNF-αexpression at the 7th day, PNS treatment group and PNS pretreatment group were the second strongest. The sham-operated group and saline group had no significant expression. There were no statistical significance difference(P>0.05) between PNS treatment group and PNS pretreatment group on animals neurobehavior score, basilar artery wall thickness and expression of TNF-α.Conclusion:1. The local inflammatory response on cerebrovascular wall may be the main reason caused by CVS after SAH, in which TNF-αplays an important role in the local inflammatory response of the cerebrovascular wall.2. PNS can effectively reduce the severity of CVS after SAH.3. The mechanism of PNS preventing and treating the CVS may be by inhibiting the expression of TNF-α.4. There is no significant difference between the effects of PNS pretreatment and PNS treatment...