Antitumor Effects Of Berberine α-hydroxy δ-decanoylethyl Sulfonate And Its Induction Of Apoptosis In B16 Cells

Objective: To investigate the antitumor effects of berberineα-hydroxyβ-decanoylethyl sulfonate (HB), and its mechanism such as induction of cell apoptosis and differentiation.Methods: 1) MTT assay was employed to determine the cytotoxicity of HB in HepG2,MGC80-3, SW480 and other cells. 2) HB was administrated to treat mouse transplanted solid tumors H22 and S180,Colon26(a human cancer)in nude mice, and the tumor growth was observed. 3) The difference of melanin content in B16 cells between HB treatment group and control group was determined by using absorption photometry. 4)Through Rhodamine 123 dyeing, agarose gel electrophoresis, Hoechst 33258 dyeing, active detection of caspase 3 and caspase 8, observe the cell apoptosis induced by HB. 5)CD44V6 expression of Colon26 tumors which was transplanted in nude mice was detected by immunohistochemical technigue, and the influence of HB was observed on it.RESULT: HB significantly inhibits the proliferation of HepG2, MGC80-3, SW480 and other cells in vitro;inhibits the growth of H22 and S180 in mice, Colon26 tumors in nude mice. Melanin content of B16 cells increase after treatment with HB. The evidences of B16 cells apoptosis induced by HB were provided, such as Rhodamine 123 fluorescence intensity obviously decreased, DNA Ladder detected, nuclei pyknosed, caspase3 and caspase 8 activity increased. CD44V6 expression was decreased in Colon26 tumors which was treated with HB.Conclusion: HB has significant antitumor activity in vitro & in vivo, and can induce differentiation and apoptosis of B16 cells, reduce CD44V6 expression in Colon26 tumors.