The Effect Of Telmisartan On The Blood Pressure,myocardium Angiotensin Converting Enzyme And Angiotensin Converting Enzyme 2 Chronic In Mice Under Intermittent Hypoxia Condition.

Objective To observe the effect of chronic intermittent hypoxia in sleep apnea syndrome patients on renin angiotensin system and the protection of angiotensin receptors blocker on cardiovascular system, so as to explore the mechanism which sleep apnea syndrome act on cardiovascular system.Methods 32 healthy male C57B6Jmice were randomly divided into 4 groups: the CIH group, the telmisartan treatment group,the air control group, and the blank control group,and 8 mice in each group.The period of chronic intermittent hypoxia last for 12weeks. Angiotensin converting enzyme(ACE),angiotensin converting enzyme2 (ACE2) and angiotensin II(AngII)in the mice cardiac myocyte were tested by immunohistochemistry. The blood pressure were measured as well.Results (1)For comparsion of levels of systolic blood pressure,it was significantly higher in the CIH group than other groups and came down after ARB treatment. It was significantly different between the CIH group(113.8125±11.59414mmHg)and the air control group(99.6875±8.31065mmHg)(P=0.004),the CIH group and the blank control group(104.6875±5.713 mmHg)(P=0.049),the CIH group and the ARB treatment group(78.625±8.90325 mmHg)(P=0.000),the ARB treatment group and the blank control group(P=0.000),the ARB treatment group and the air control group(P=0.000),the ARB treatment group and the CIH group(P=0.000).There was no differences between the air control group and the blank control group . (2)For comparsion of levels of diastolic blood pressure,it was significantly lower than other groups after ARB treatment. It was significantly different between the ARB treatment group(52.5±10.78359 mmHg)the blank control group(71.1875±7.17604 mmHg)(P=0.000),the ARB treatment group and the air control group(66.5625±6.64636 mmHg)(P=0.003),the ARB treatment group and the CIH group(71.1875± 9.14931mmHg)(P=0.000). There was no differences between the air control group and the blank control group(P=0.291),the blank control group and the CIH group(P=1.000),the air control group and and the CIH group(P=0.291). (3)For comparsion of levels of ACE immunohistochemisty in mice'hearts,it was significantly higher in the CIH group than other groups and came down after ARB treatment.. It was significantly different between the CIH group(0.034900±0.0129777)and the air control group(0.005238±0.0025928)(P=0.000),the CIH group and the blank control group(0.004738±0.0025618)(P=0.000),the ARB treatment group(0.025100±0.0061184)and the air control group(P=0.000),the ARB treatment group and the blank control group(P=0.000).But there was no significant differences between the CIH group and the ARB treatment group(P=0.161),the air control group and the blank control group(P=0.798). (4)For comparsion of levels of ACE2 immunohistochemisty in mice'hearts,it was highest in the ARB treatment group,followed by the CIH group . It was significantly different between the CIH group(0.052200±0.0191767)and the air control group(0.029313±0.0135514)(P=0.008),the CIH group and the blank control group(0.017488±0.0131051)(P=0.000),the CIH group and the ARB treatment group(0.072850±0.0175356)(P=0.016),the ARB treatment group and the air control group(P=0.000),the ARB treatment group and the blank control group,the ARB treatment group and the CIH group(P=0.016). There was no significant differences between the air control group and the blank control group(P=0.152). (5)For comparsion of levels of AngII immunohistochemisty in mice'hearts,it was highest in the ARB treatment group,followed by the CIH group. It was significantly different between the ARB treatment group (0.149025±0.0216644)and the CIH group(0.095922±0.0328323)(P=0.004),the ARB treatment group and the air control group(0.020684±0.0107095)(P=0.000),the ARB treatment group and the blank control group(0.015019±0.0069788) (P=0.000),the CIH group and the ARB treatment group(P=0.004),the CIH group and the air control group(P=0.000),the CIH group and the blank control group(P=0.000). There was no significant differences between the air control group and the blank control group(P=0.248).Conclusion(1)This experiment confirmed that chronic intermittent hypoxia might induce high systolic blood pressure and the expression of ACE,ACE2 and AngII in mice hearts.(2)After ARB treatment, the expression of ACE2 and AngII in mice hearts got higher and the blood pressure came down.