The Surface Marker Protein PCX Of Podocyte In The Expression Of Chronic Kidney Disease

ObjectiveTo detect the expression level of podocyte surface marker protein PCX in the urine of patients with Chronic kidney disease, and analyse the relationship among the serum creatinine, urine trace protein (α1-MG, TRF, ALB), Ccr and urinary total protein. To explore whether PCX could be a indicator of glomerular injury.MethodsUrinary samples were obtained from 62 patients with chronic kidney disease from March to December in 2009, and 22 healthy volunteers as normal control group were collected at the First Affiliated Hospital of Suzhou University. According to the NKF-K/DOQI of chronic kidney disease, 62 patients were divided into four groups: 28 cases in CKD2 group(A group), 11 cases in CKD3 group (B group), 12 cases in CKD4 group (C group), 11 casses in CKD5 group (D group). The middle period of the first morning urine from patients of chronic kidney disease was about 5ml. We used enzyme-linked immunosorbent assay (ELISA) to detect urinary podocyte surface marker protein PCX in patients of chronic kidney disease and healthy volunteers, and to investigate the relation between PCX, Scr, urinary trace protein (α1-MG, TRF, ALB), Ccr and urinary total protein.Results1.The expression level of podocyte surface maker protein PCX in patients of chronic kidney disease (23.43±19.64) was higher than the normal (0.65±0.42).2.The expression level of PCX in patients of chronic kidney disease was significantly difference at the different stage of renal injury (P<0.01).3.ALB,α1-MG and TRF often used to reflect renal damage indicators, there was no significant difference between the group A and group B (P> 0.05), there was significant difference between the group C and group D (P <0.01).4.There was a certain connection between PCX and urinary total protein in kidney disease (r = 0.082, P <0.05).Conclusion PCX may be involved in the process of kidney disease and as a indicator of kidney damage.1.The PCX of Nephropathy was significantly higher than normal, suggesting that kidney damage may be related to PCX.2.PCX were reduced with the progress of kidney, In the assessment of renal lesions PCX may be superior to urine protein.3.There was a certain connection between podocyte surface marker protein PCX and urinary total protein. in kidney disease.