Epidemiological Study On High Level Gentamicin Resistant Enterococcus Spp Of Animal Source

AbstractIn recent years, growing attention has been focused on the High level gentamicin resistant (HLGR) Enterococcus spp. Our study aimed at investigating on the epidemiology of HLGR Enterococcus spp and analyzing the affecting factors which could induce the form of resistance.307 strains of Enterococcus spp from 3 pig farms of different regions of Fujian province were isolated and 179 strains of Enterococcus spp from one chicken farm were also isolated in two years.163 and 78 strains of HLGR Enterococcus spp were isolated by selection test. Antibiotic susceptibility test was carried by micro-broth-dilution method according to Clinical and Laboratory Standards Institute (CLSI). The results showed that the isolated rate of HLGR Enterococcus spp from pig farms was 53.09%, the resistant rate of these bacterial to oxytetracycline, erythrocin, vancocin, ampicillin, ciprofloxacin and chloramphenicol was 99.38%; 100%; 0%; 1.23%; 59.51%; 79.14%, respectively. There was no significantly difference between newly build pig farm and old pig farm. HLGR Enterococcus spp isolated from weaned pig and sucking pig has the highest resistant rate to six antibiotics, then Lactation Sow, pregnant sow and fattening pig, and hog has the lowest resistant rate.88 and 91 strains of Enterococcus spp were isolated in 2007 and 2008 respectively, there were 35 and 43 strains of HLGR Enterococcus spp isolated and the isolated rate was 39.77% and 41.25%, respectively. The resistant rate of those bacterial isolated in 2007 to oxytetracycline, erythrocin, vancocin, ampicillin, ciprofloxacin and chloramphenicol was 100%,100%,2.85%,8.57%,88,57%,74.29%, respectively. The resistant rate of those bacterial isolated in 2008 to oxytetracycline, erythrocin, vancocin, ampicillin, ciprofloxacin and chloramphenicol was 100%,100%,4.65%,6.98%,81.40%,65.12%, respectively. There were no significant different between two years. The results indicated that HLGR Enterococcus spp has highly resistant rate to many antibiotics and there were vancocin resistant HLGR Enterococcus spp isolated. Some measurements must be taken to prevent the increasing of resistance of HLGR Enterococcus spp to other antibiotics.