Study On Therapeutic Efficacy Of Hemorrhagic Rats Of Different HCT Resuscitation Fludis

Objective The effects were investigated of heating on the toleration of blood loss in rat hemorrhagic shock models. Criterion of suitable animals and the effects of four different resuscitation fluids on the hemorrhagic shock rats were investigated.Methods All rats were anesthetized with urethane by muscle injection. Then the volume～controlled hemorrhagic model was prepared and the performed period was maintained 60 minutes. During the 60-min shock period, the survival number and the body temperature were recorded at T0, T20, T30 and T40, which stand for the blood loss reached 0, 20%, 30% and 40% of the total blood volume.The hemorrhagic rats were infused different resuscitation fluids. The rats'physiological conditions and blood biochemistry index were observed after resuscitation in 90 min.Results The survival rate was higher in the heating group than that of non-heating group (p<0.05). After statistic analysis, the physiologic index of PaO2, PaCO₂ and MAP were found significant effects on blood loss toleration (p<0.05), but the pH and HCT were not significant effects on it (p>0.05). Four resuscitation fluids all have the therapeutic efficacy in hemorrhagic rats. There are many significant differences on the several indexes between the resuscitation in 90 min with the shock in which HCT is 40% and 10% resuscitation fluids. Conclusion Heating can improve the toleration of blood loss and increase the survival rate in the rat hemorrhagic shock models. We conclude that PaO2, PaCO₂ and MAP may be the suitable criterion for hemorrhagic rat shock model. The resuscitation fluids which HCT is 40% and 10% could correct acid～base metabolic disturbances and restore carotid artery blood flow rate effectively. In the maintenace of MAP and temperature which is basic vital signs for animal and regulateing microcirculation, the resuscitation fluids which HCT is 40% is the bast one of the therapeutic efficacy. In the resuscitation effect of adjusting oxygen metabolic, the fluids which contain RBC is batter than the plasma fluid, and the therapeutic efficacy is related to HCT.