Expression Of Cyclin E ,P27 And Ki-67 Proteins In Intracranial Tumor

OBJECTIVE: To study the expression of cyclin E,p27 and Ki-67 proteins in intracranial tumor and their relationships. METHODS: With intracranial meningiomas and gliomas, as representatives of benign and malignant, immunohistochemical method was used to detect expression of cyclin E, p27 and Ki-67 proteins in 41 cases of meningioma, 47 cases of gliomas and 20 cases of normal brain. RESULTS: Cyclin E was not expressed in normal brain tissues. The high positive rate of cyclinE protein was14.63% in meningioma, 50.00% in gradeⅠ～Ⅱof gliomas and 92.59% in gradeⅢ～Ⅳof gliomas, there was significant difference (P<0.05) between them, and with the advancing of pathological grades, the PU of cyclin E increased accordingly. Similar to cyclin E, the high positive rate of p27 protein was 95.02% in normal brain tissues,80.49% in meningioma, 55.00% in gradeⅠ～Ⅱof gliomas and 33.33% in gradeⅢ～Ⅳof gliomas, there was significant difference (P<0.05) between them. was not expressed in normal brain tissues. With the advancing of pathological grades, and with the advancing of pathological grades, the PU of cyclin E decreased accordingly. Ki-67 was not expressed in normal brain tissues. The high positive rate of Ki-67 protein was9.76% in meningioma, 45.00% in gradeⅠ～Ⅱof gliomas and 66.67% in gradeⅢ～Ⅳof gliomas, there was significant difference (P<0.05) between them, and with the advancing of pathological grades, the PU of cyclin E increased accordingly. There was negative correlation in staining intensity between cyclin E and p27 proteins in intracranial tumor (r=-0.495, P < 0.05). There was no correlation in staining intensity between p27 and Ki-67 proteins in intracranial tumor (r=0.705, P < 0.05). There was positive correlation in staining intensity between cyclin E and Ki-67 proteins in intracranial tumor (r=-0.595, P < 0.05). CONCLUSION: The expression of cyclin E and Ki-67 is higher in gliomas and lower meningiomas, not expressed in normal brain tissues. P27 is lower in gliomas and higher in meningiomas and normal brain tissues. It is suggested that cyclin E ,p27 and Ki-67 proteins may play an important role in intracranial tumor.