Establishment Of Bacterial Ventriculitis In Rabbits And Antibiotic Intervention Of Different Ways

Objective Intracranial infection is one of the relatively serious complications in neurosurgery, ventriculitis is the most severe type, which strongly influence the prognosis. In view of the bad treatment outcome, this study will to investigate the establishment of rabbit model of bacterial ventriculitis and then compare the influence of the different antibiotics route of administration combined with mannitol.Methods Thirty-six adult healthy New Zealand white rabbits were randomly divided into model group (group A), operation control group (group B) and normal control group (group C). There were 12 rabbits in each group. The lateral ventricles of the rabbits in model group were inoculated with 0.5ml staphylococcus epidermidis suspension, which the concentration was 1×106CFU/ml. In the operation control group, 0.5ml 0.9% sodium chloride was injected and in the normal control group no operation was done. After animal model was successfully done, rectal temperature was monitored, blood and CSF were checked, then 12 hours later all animals were killed and brain tissue was observed for pathology study. Fifty bacterial ventriculitis model rabbits were randomly divided into intravein group (groupⅠ), intra-artery group (groupⅡ), combined intravein group (groupⅢ), combined intra-artery group (groupⅣ) and 0.9% sodium chloride control group (group V). There were 10 rabbits in each group. Treatment (once every 12 hours, total 6 times) began after blood and CSF samples were taken. For groupⅠ, SULPERAZON(?) was injected via vein. For groupⅡ, SULPERAZON(?) was injected via internal carotid artery. For groupⅢ, SULPERAZON(?) was injected via vein after mannitol was injected. For group IV, SULPERAZON(?) was injected via internal carotid artery after mannitol was injected. For group V, 0.9% sodium chloride was injected. Rectal temperature and rate of survival time were monitored. The CSF concentration of cefoperazone was measured by HPLC. Blood and CSF were checked.Results In group A, the rectal temperature, total leukocyte count, neutrophils percentage of blood, protein level, cells and leukocyte count of CSF were increased significantly, glucose level, chloride level of CSF were decreased significantly, blood bacterial culture and CSF bacterial culture were negative and positive, respectively . In group B and C, those indexes had no significant change, blood bacterial culture and CSF bacterial culture were both negative. In group A, pathological observation showed neutrophile granulocyte infiltration in subependymal and in group B and C observation showed normal. The CSF concentration of cefoperazone in group IV is the highest and then group II, group III, group I, respectively. There were significant difference between group IV and group II, III, I. The rate of survival time in group IV is higher than the group III after 96 hours'treatment and the animals in group I, group II and group V were all dead. The rectal temperature, leukocyte count of blood, and leukocyte count of CSF were significant difference between group IV and group III after 96 hours'treatment.Conclusion 1. The bacterial ventriculitis model established in this study meets the clinic manifestations and histological changes in clinic, this study offers a better model for the research of bacterial ventriculitis.2. Treatment with SULPERAZON? through internal carotid artery injection combined with mannitol was better than the way in intravein, intra-artery, combined intravenous injection.