| OBJECTIVE: This study made a model of pulmonary thromboembolism based on the hyperhomocysteimemia in the rabbit, the change of endothelin-1 (ET-1), nitrogen monoxide (NO), lipoprotein a (Lpa), D-dimer were measured before and after the establishment of the model of pulmonary thromboembolism (PTE) . In order to discuss the correlation of the homocysteine (Hcy) and PTE.METHODS : Divided Thirty New Zealand white rabbits into two groups: homocysteine model group (Hcy group) and General group. The Hcy were measured before feeding to two groups to get the 95% confidence interval (12.81-14.31umol/L). Hhcy model was established by the method of raising rabbits with food 2% methionine, general group was raised normally. after eight weeks. The Hcy was measured once again, we found the Hcy of Hcy group was Significantly higher and general group was no change. PTE models were established by injection of auto-blood clots into the jugular vein of two groups rabbits. The changes of the contents of D-dimer,ET-1, NO, Lpa and blood gas analysis in plasma were measured in different time before and after PTE in rabbits, and for pulmonary perfusion imaging. The pathological study of anatomy for lung was performed to observe :1. the distribution of thrombosis ;2. the abnormal formation of pulmonary artery and femoral vein.RESULTS: The serum levels of Lpa, NO were no significant different between the two groups rabbits before the embolization. The level of NO in blood was significantly decreased at 2 hours after embolization in two groups, compared with pre-embolization. Lpa was elevated significantly at 24 hours after embolization(P﹤0.05). Hcy group change more obvious than the general group. There was statistical significance between the two groups(t= -2.995, -4.544, P﹤0.05).The plasma levels of ET-1 and d-dimer increased singificantly at 2h after embolism in two groups. There was statistical significance between the two groups (t= -7.658, -2.716, P﹤0.015).Pathological results:①Generally seen: the injected blood clots thrombosis in the pulmonary ateries in two groups,There were 88 clots,less than the number of clots injected 111. The clots of general group were observed in the following paragraphs, with congestion-based,but the clots of Hcy group were mainly seen pulmonary trunk and 85% of the pulmonary infarction occurs.②HE staining can be seen under the microscope: there was a remarkable expansion at the embolization site, part of the intima can be seen in the growth of the thrombus. The pulmonary artery, aortic lumen of Hcy group were significantly thinner, narrower and wall thickening than general group. Femoral vein was no significant difference between two groups. These demonstrate that Hcy may be little effect on venous.Lung perfusion imaging: after PTE, pulmonary perfusion imaging positive rate of two groups was 75%, Hcy group rabbits were more obvious filling defect. Blood gas analysis: The two groups after embolization were measured PH, PO2, PCO2. The PO2 of Hcy group significantly decreased, compared with general group, (P<0.05) but PH and PCO2 were no significant difference between two groups. (P>0.05)CONCLUSION:1. The PTE was more easily occurred based on the Hcy, and it was serious, thorough and poor prognosis.2. Hcy can promote the occurrence of PTE.3. Pulmonary perfusion imaging combined serological markers for early diagnosis of pulmonary embolism has a significant value.
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