| ObjectiveTo investigate distribution of Interferon Regulatory Factor 5(IRF-5) Gene in the Rheumatoid Arthritis (RA) patients and controls in Han Chinese population.To determine whether Interferon Regulatory Factor 5 Gene T allele is associated with the susceptility,clinical manifestation and severty of RA.Methods1. IRF-5 rs2004640 is genotyped by polymerase chain reaction- ligase detection reaction(PCR-LDR) in 139 patients who fulfilled the 1987 American College of Rheumatology(ACR) and 152 controls.All patients are tested for the presence of autoantibodies against cyclic citrullinated peptides(anti-CCP) by ELISA.Chi-square test were used to compare the genotypes and allele frequencies in the RA patients versus the controls.The SHEsis software was used to assesse the Hardy-Weinberg quilibrium of the genotypes.2. Record the age, gender ,onset age,history,extro-articular manifestation(EAM),joint destruction and DAS28 of 139 patients. Rheumatoid factor(RF) was detected by latex agglutination. Erythrocyte sedimentation rate(ESR)was detected by Wen,s method. Chi-square test and T test were used to compare the T allele frequencies in the RA patients of age, gender ,onset age,history,joint destruction , DAS28,RF,ESR and EAM. Results1. The genotype frequencies of IRF-5 rs2004640 in anti-CCP negative RA patients were statistically different from those in controls(χ~2=6.714,P=0.035). The frequencies of TT genotype(13.5%vs5.9 %) and T/G genotype(40.4%vs28.9 %) were higher and frequencies of GG genotype(46.2%vs65.1 %) were lower in anti-CCP negative RA than in controls. No significant differences of genotype frequencies were observed between all RA and controls (χ~2=4.808,Ρ=0.090). No significant differences of genotype frequencies were observed between anti-CCP positive and anti-CCP negative RA patients (χ~2=2.935P=0.231). No significant differences of genotype frequencies were observed between anti-CCP positive and controls(χ~2=2.082 ,P=0.353).2. The T alleles and G alleles frequencies of IRF-5 rs2004640 in anti-CCP negative RA patients were statistically different from those in controls(χ~2=6.803,P=0.009). The frequencies of T alleles(33.7%vs24.7 %) were higher and frequencies of G alleles(66.3%vs75.3 %) were lower in anti-CCP negative RA than in controls. The T alleles and G alleles frequencies of IRF-5 rs2004640 in RA patients were statistically different from those in controls(χ~2=4.257,P=0.039). The frequencies of T alleles (28.1%vs20.4 %) were higher and frequencies of G alleles (71.9%vs79.6 %)were lower in RA than in controls. No significant differences of alleles frequencies were observed between anti-CCP positive and controls(χ~2=0.965,P=0.326). No significant differences of alleles frequencies were observed between anti-CCP positive and anti-CCP negative RA patients(χ~2=2.154,P=0.142).3. There was no difference between the patients with T allele and patients no with T allele in age, gender ,onset age,history,RF,ESR .We detect significant differences between the patients with T allele and patients no with T allele in EAM(χ~2=18.931,P=0.000). There was difference between the patients with T allele and patients no with T allele in joint destruction(χ~2=5.618,P=0.018). There was difference between the patients with A allele and patients no with A allele in DAS28(χ~2=6.218,P=0.013).Conclusions1. TT and T/G genotype of IRF-5 rs2004640 is correlated with anti-CCP negative RA patients in Han population of Tangshan district in China.2. T alleles of IRF-5 rs2004640 is correlated with the susceptibility of RA.3. T alleles of IRF-5 rs2004640 has influence on activity and severity of RA. |
