| ObjectiveOsteoarthritis is one of the most popular diseases,without significant difference in race and district. The degeneration of cartilage is the most important factor in osteoarthritis. Recently it is reported that MMPs plays crucial action, the degeneration of MMPs in matrix leading to the degration, reduction and destruction of cartilage. IL-1βand other pro-inflammatory cytokines can enhance the expression of MMPs through signal pathways such as MAPK,NFκB and so on.It is recently reported that there is some association between wnt/β-catenin pathway and osteoarthritis.β-catenin is a major component in this pathway, and the over expression of pathway mediated byβ-catenin leads to the destruction of cartilage. sFRP-1 is a kind of antagon of the wnt/β-catenin, loss in quantity or function leading to the over activation of the pathway, then causing the reduction in type II collagen, proteoglycan as well as related GAGs, all of which promote the occurance of OA.Calcitonin, a public antirebsorbent , is a kind of medicine for osteoporosis. A lot of reports recently have demonstrated that calcitonin can promote the synthesis of proteoglycan and cell proliferation, which is suggested by vivo through results of histomorphology. Nowdays calcitonin ,as a potential medicine for OA, have been payed more attention, and its protection for chondrocytes has become focus, but it is not clear about the molecular mechanism. This article aims at protection of calcitonin to chondrocytes of osteoarthritis through detectingβ-catenin and sFRP-1. MethodsThe Japanese rabbits'chondrocytes are designed, intervened by another medicine, and then have an observation of the action of calcitonin in chondrocytes. The test is divided into three groups including the blank group, the IL-1βgroup and the calcitonin group. The expression ofβ-catenin is observed by immunocytochemistry andβ-catenin and sFRP-1 are detected by Realtime PCR. Results were analyzed statistically by ANOVA.Results:1.The results of immunocytochemistry: the IOD ofβ-catenin are respectively in the calcitonin group 3.086±0.590, the IL-1βgroup 10.163±0.969, the blank group 6.628±0.808; There is difference after statistics( P<0.05) compared the calcitonin group to the IL-1βgroup; the same as the calcitonin group and the blank group; and there is also difference between the IL-1βgroup and the blank group (P<0.05) after statistics.2.The results of Realtime PCR: the amount ofβ-catenin mRNA are respectively in the calcitonin group 4.945±1.302, the IL-1βgroup 9.513±0.137, the blank group 1±0; the amount ofβ-catenin mRNA is much lower in the calcitonin group than the IL-1βgroup; There is difference after statistics( P<0.05) compared the calcitonin group to the IL-1βgroup ; It is much higher in the IL-1βgroup than the blank group; and there is also difference between the IL-1βgroup and the blank group (P<0.05) after statistics. The amount of sFRP-1 mRNA in the calcitonin group 2.917±0.580, the IL-1βgroup 0.285±0.059, the blank group 1±0; It shows that there is statistic difference (P<0.05) between the calcitonin group and the IL-1βgroup; and sFRP-1 mRNA in the IL-1βgroup is much lower than the blank group, showing the statistic meaning( P<0.05) ; meanwhile the sFRP-1 mRNA in the calcitonin group seems to be much higher than the blank group, and it proves to be statistic meaning (P<0.05) .Conclusion The expression ofβ-catenin can be inhibited and sFRP-1 be promoted in those chondrocytes acted by calcitonin compared to the the blank control group, the negative group of IL-1β, which stop the destruction of chondrocytes. That demonstrate the function of protection of calcitonin for chondrocytes of OA.
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