Relationship Between Angiotensin â…¡ Type 1 Receptor Gene A1166C Polymorphism And Chronic Heart Failure

Objective:To investigate the relationship of angiotensin II type1 receptor gene A1166C polymorphism and chronic heart failure. To probe into the mechanism of Chronic Heart Failure on genetic level and to provide basis for containing the progression of heart failure.Method: A case-control study was carried out in chronic heart subjects. All the eligible subjects were enrolled into the study after signing informed consent. From December 2005 to December 2006, in Kailuan Hospital we selected 135 chronic heart failure patients as case group according to standard and meanwhile 121 person were identified as control group who examined at the Kailuan Hospital healthy physical examination center as the same time with the same nationality, the same homogeneity, the same housing conditions, the sex and age approximately matched with the case subjects. Polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) were used to detect angiotensin II type 1 receptor gene A1166C polymorphisms of the case group and control group. BUN, Cr, TG, TC, Glu, HDL-C and so on biochemistry target were detected by automatic biochemical analyzer.The database was set up by Excel and analyzed by using the SPSS Statis- tics software after checking reorganization unification code with Excel. Two sample mean value comparison uses the T-test, many sample mean value comparison uses the single factor variance analysis. The genotype and the allele frequency comparison use Chi-squre examines between case group and control group. P<0.05 has statistics significance.Results: 1. A total of 256 eligible subjects was enrolled into the trials, among which six subjects was eliminated because of failure of PCR . At last, the total of 250 subjects were surveyed. Among these test,129 subjects were selected as case group, 89 males and 40 females, the average age was 67 years old (67.47±11.28), and 121 subjects were selected as control group,86 males and 35 females, the average age was 66 years old (65.70±8.04). There was no significant difference between gender and age constitution of case and control group. This study has good comparability. Three genotypes (AA AC CC) were detected of the subjects. The information is representative because genotype distribution of AT₁R A1166C was consistent with Hardy-Weinberg genetic equilibrium. Combine AC and CC together because the frequency of CC of angiotensin II type 1 receptor was too rare. There was no significant difference of the frequency of AT₁R A1166C genotype and allele in the case group and control group(P>0.05).2. Regardless of the case group or the control group, the comparison of BUN Cr TG TC Glu and HDL-C had no significant difference during three genotypes( P>0.05).3. Divide case group and control group into four parts according to different sex. There was no significant difference of the frequency of AT₁R A1166C genotype and allele between male and female in the control group(P>0.05). No significant difference of the frequency of AT₁R A1166C genotype and allele was found between male case and male control group(P>0.05). No significant difference of the frequency of AT₁R A1166C genotype and allele was found between female case and female control group (P>0.05).4. Select the patients caused by coronary heart disease as one group and essential hypertension as other group, and 121 person as control group. Age, and sex constitution compared has no significance difference of three groups. There are no significant difference in the three groups of the comparison of genotype and allele frequency (P>0.05). Conclusions1 .Angiotensin II type 1 receptor gene A1166C polymorphism was not associated with chronic heart failure2. Angiotensin II type 1 receptor gene A1166C polymorphism was not associated with chronic heart failure caused by coronary heart disease.3. Angiotensin II type 1 receptor gene A1166C polymorphism was not associated with chronic heart failure caused by essential hypertension.