Establishment And Evaluative Of Anger-out And Anger-in Induced By Social Isolation And Resident Intruder Stress

Objective: Our aims of this study are to introduce rat model and research evaluative method of anger-out/in emotional reaction, to investigate changes of neurotransmitter and hormone and to initially explore the mechanism of rat model as well as effect of medicine.Methods: Model rats with anger-out/in emotional reaction are induced by social isolation paradigm plus resident intruder stress, after that we distinguished the difference between angry-out and anger-in by aggressive behavior test, supplemented by open-field test and sugar preference test, subsequently examine the stability of the modeling method, and then intervened with Jingqianping (Jingqianshu) granule. After model establishment,we killed the rats by decapitation,collected serum and dissected different brain regions(prefrontal cortex, parietal cortex, hippocampus and hypothalamus), detected ACTH, Cor, T,NE,DA,5-HT and GABA content with radioimmunoassay and high-performance liquid chromatography .Results:⑴Compared with the normal group, the body weight of high aggressive(HA) and low aggressive(LA) rats lessened significantly(P<0.001),while drenal glands coefficient of HA and LA rats'reduced (P<0.01).⑵In aggressive behavior test, composite aggressive counts of HA rats were significantly higher than LA(P<0.001, P<0.001),latent time of attack were lower(P<0.001, P<0.001);In open-field experiment HA/LA rats'total scores were also higher than controlled (P<0.01), and HA rats'higher than LA's, while comparison result of sugar preference coefficients contrary to it (P<0.01);⑶Compared with anger-in group, Cor contents in serum of anger-out model rats descended notably(P<0.01),while NE contents increased(P<0.05); After administrated with Jingqianping (Jingqianshu) granule, abnormal fluctuations of 5-HT are obviously corrected between anger-out and anger-in (P<0.05).Compared with the control group, NE contents in hypothalamus, parietal region and hippocampus of anger model decreased (P<0.001), while DA contents in hypothalamus, and then restored after administration. Unlikely, DA contents in prefrontal cortex decreased (P<0.01), but increased in hypothalamus, they could both be corrected after administration. DA contents of anger-out model rats in prefrontal cortex and hippocampus were lower than anger-in group (P<0.001).Compared with the control group, 5-HT contents in hypothalamus of anger model rats degraded significantly (P<0.001), while increased in prefrontal cortex and decreased in hypothalamus, the abnormality disappeared after administration. 5-HT contents of anger-out model group in hypothalamus were obviously higher than anger-in group (P<0.05).Conclusion: The anger-out/in emotional reaction rat model can be introduced by the method of social isolation plus resident intruder stress. Modeling method was reliable and mature with a better credibility and repeatability; Adopting method aggressive behavior could distinguish anger-out/in belongings of rats'model clearly.Parts of the microcosmic mechanism were disclosed: anger-out emotion maybe related to NE, 5-HT level descending in hypothalamus, DA level descending in prefrontal cortex, parietal cortex and hippocampus, DA level increasing hypothalamus;while anger-in emotion maybe related to NE level increasing in parietal cortex and hippocampus,DA level increasing in hypothalamus, 5-HT level increasing in prefrontal cortex, 5-HT,NE level descending in hypothalamus, DA level descending in prefrontal cortex. We can assume that Jingqianping (Jingqianshu) granule play the role of pharmacological effects by corrected those indicators and its possible function location is different cerebral regions.