The Change Of Substance P (SP) In Serum, Spinal Cord And Synovium Of Rabbit After Performing Femoral Nerve Blocking

Objective:To explore analgesia effect on knee pain through observing the change of Substance P (SP) in serum,spinal cord and synovium of rabbit after performing femoral nerve blocking.Method:30 rabbits were divided into three groups, normal (A).contrast(B) and experiment group(C).B and C group were made into the model of arthritis, and then C group was performed consistent femoral nerve blocking with ropivacaine for one week. The change of SP in serum, spinal cord and synovium of habit in all groups were detected through the technology of ELISA and immunohistochemistry technology. Result:The mean concentration value of SP in serum of A group is 29.34±1.43 meanwhile B group is 21.56±6.94,C group is 28.66±2.92.There is significant difference between A and B and so is between C and B group p<0.01. The mean OD value of SP in spinal cord of A group is 9.60±2.79 meanwhile B is 24.46±4.78, C is21.56±6.94, it hasn't been founded any significant difference between B and C group. The positive result of SP wasn't detected in synovial specimens.Conclusion:From the result that the release of Substance P in serum reduces after femoral blocking but without any change in spinal cord, we can conclude that the analgesia effect of femoral nerve blocking isn't complete.