The Effect Of Low-frequency Ultrasound Combined With Nanofibrous Membrane On Bone Defect

Background:At present, the regeneration of the large bone defect presents a major challenge in clinical practice. The biomimetic material and the low-frequency ultrasound have been shown to augment the bone healing in many animal experimental settings. Nanofibrous membrane is a good biomaterial in terms of ability to induce MSCs (mesenchymal stem cell) osteo-differentiation and sound biocompatibility; while low frequency ultrasound has been a feasible, non-invasive, and economic modality for the bone fracture. We present the study to test the hypothesis that ultrasound improve the efficacy of the nanofibrous membrane to heal bone defect.Objective:The aim of our study is to observe the low-frequency ultrasound combined with nanofibrous membrane in repairing defects of long bone.Methods:Large tibia bony defects were created in 12 adult male New Zealand rabbits, then the rabbits were divided into tow groups. Group A (n=8) the left tibia treated with nanofibrous membrane reinforced with ultrasound, the right tibia treated with nanofibrous alone. Group B (n=4) the left tibia treated with nanofibrous membrane and the right tibia treated with neither nanofibrous membrane nor low frequency ultrasound. Three and six weeks after operation, the bone defect healing were accessed for radiological and histological analysis.Results:The modality that low-frequency ultrasound combined with nanofibrous membrane induce more quantity of new-formed bone compared with the nanofibrous membrane, and the bony defect treated with low-frequency ultrasound combined with nanofibrous membrane showed more organized.Conclusion:Nanofibrous membrane reinforced by low-frequency ultrasound could induce the regeneration of bony defect and bone remolding.