| Background and Purpose Pancreatic cancer is a common malignancy with onset hidden and the rapid development,and lack of an effective system for early diagnosis and treatment. Its poor Prognosis is to encourage people to seek the new method of diagnosis and treatment. The main feature of pancreatic cancer is local invasion and early metastasis. The mechanism of it is unclear, which involvs a large number of biologically active molecules. In depth study of these molecules is beneficial to reveal the mechanisms of invasion and cancer, to find new and effective treatment. It is to improve the prognosis and treatments of pancreatic cancer.Trophinin is a unique cell adhesion molecules expressed in human trophoblast. Previous research has confirmed that it plays an important role in the process of embryo implantation. Recent studies demonstrated that it is in a variety of malignant cells with up-regulated expression and promoted more invasiveness of cancer cells in vitro, suggesting it is closely related to tumor invasion and metastasis, however, its involvement of pancreatic cancer has not been reported. This experiment is intended to study the expression of Trophinin gene and its protein, to analyze its involvement of pancreatic cancer clinical stage, histological grade, patient gender, age, CEA, CA199, CA125, and other clinicopathological data.Methods Surgical resection specimens were referred from the Second Affiliated Hospital of Chongqing Medical University from January 2007 to April 2009. Pancreatic cancer and adjacent tissues was 32 cases, 10 cases of normal pancreatic tissue. Adjacent noncancerous tissues were less than 2cm from pancreatic cancer tissues, and cancer cells were not seen under a microscope. 10 cases of normal pancreatic tissues samples were selected from the normal pancreas with common bile duct cancer and pancreas duodenal carcinoma excision. Each section is stained with HE, Trophinin protein immunohistochemistry SP method and westem blot test. Patients after operation were followed up within 24 months, and the data was analyzed in conjunction with experimental results and statistical analysis.Results Trophinin protein is mainly expressed in nucleus and nuclear membrane of pancreatic cancer cells, small amount is expressed in the cytoplasm. Trophinin protein expression in pancreatic tissues are 81.3% (26/32), in adjacent noncancerous tissues 40.6% (13 / 32), in normal pancreatic tissue 10.0%(1/10). Westem blot detection protein level of Trophinin are followed by increased in normal pancreatic tissues and adjacent noncancerous tissues and pancreatic cancer tissues, were followed by increased in stages I, II, III, The differences were statistically significant. Trophinin protein expression is not concerned in patients'gender, age, tumor location, CEA, CA199 and CA125; however it is closely involved in clinical stage, tumor differentiation and neural invasion. Neural invasion is found in nine patients, eight are revealed that Trophinin protein expression is strongly positive, moreover, in poorly differentiated pancreatic cancer is more, and stage III was significantly stronger than stage I, II. Follow up of patients: The survival of patients with high expression of Trophinin within 30 months was significantly less than those with moderate, low and negative expression of Trophinin.Conclusion 1.Trophinin protein expression in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissue and adjacent noncancerous tissue.2. Trophinin protein expression was not concerned in patients'gender, age, tumor location, CEA, CA199 and CA125; however it was closely involved in clinical stage, tumor differentiation and neural invasion. indicating that Trophinin plays an important role in the development of pancreatic cancer.3. Trophinin strong expression is poor prognosis. Which can be served as one of the molecular targets of the pancreatic cancer clinical prognosis.
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