The Expression Of TRAF6 In Human Liver Cancer Cells And Its Mechanism

Objective: To explore the expression of essential proteins of Toll-like receptor (TLR) signaling pathway in the human Hepatoma cell line QGY. To understand the effect of Tumor necrosis factor receptor-associates factor-6 (TRAF6) on tumorgennesis of hepatocellular carcinoma (HCC). In order to investigate the immune regulatory mechanisms of TRAF6 in the human liver cancer. It provide a theory evidence for the full development and application of TRAF6.Method: The expression of the essential genes of Toll-like receptor(TLR) signaling pathway in human normal hepatic cell line (LO2) and human liver cancer cell line(QGY) was determined by RT-PCR,. The protein expression of TRAF6 was determined by Western blot. The expression of TRAF6 in QGY cells was depleted by using the short interfering RNA (siRNA). After the interference of siRNA, We observed QGY cells of normal control group, TRAF6-FAM-siRNA group and FAM group after transfection under fluorescence microscope. The effect of siRNA of TRAF6 was ensured by RT-PCR and Western blot. The activity of QGY cells after depletion by using the short interfering RNA (siRNA) was examined by MTT assay.Result: 1. Compared to LO2 cells, the gene expressions of MAL and TRAF-6 were significantly increased in QGY cells(p<0.05)whereas the gene expressions of TRAM and TRIF have little changes. The expression of TRAF6 protein of QGY cells was significantly higher than LO2 cell. 2. six hours After interference of FAM-TRAF6-siRNA, we observed the cells under fluorescence microscope, and found effective siRNA transfection was well. And the gene and protein expression of TRAF6 was significantly decreased in QGY cells after interferrence of FAM-TRAF6-siRNA. 3. Cells proliferation was markedly inhibited in the QGY cells which was treated by TRAF6-siRNA.Conclusion: Compared to the human normal liver cells LO2, the expression of MAL and TRAF6, which are the essential proteins of TLR signaling pathway in liver cancer cells, was up-regulated. In the human liver cancer, TRAF6 plays essential role in promoting proliferation, which was expected to provide new molecular targets for targeted therapy of primary liver cancer. It can improve the validity of targeted therapy and provide a new theoretical basis and a new direction for clinical tumor therapy.