Expression Of S100A7 In Retina Pigment Epithelial Cells Cltured In Vitro

Purpose 1 To grasp the human retina pigment epithelium cells (ARPE-19)culture technology ; 2 To determine the expression and distribution of S100A7 in retinal pigment epithelial cell (RPE);3 To discuss the effects of S100A7 on RPE proliferation. To investigate the effects of S100A7 on ARPE-19 proliferation.Method 1 ARPE-19 cells were obtained from Chongqing Key Laboratory of Ophthalmology, and then continuous cell cultured. 2 The 3rd to 6th generations of cells were used in this study ,immunofluorescence technique was used to detect the expression and distribution of S100A7 in ARPE-19. 3 We extracted ARPE-19 total protein and detected the expression of S100A7 in ARPE-19 by western blot technique. 4 ARPE-19 were plated into 96-well cell culture plate and treated with S100A7 antibody, ARPE-19 proliferation were analyzed using the MTT assay ,which identified S100A7 preliminary function on ARPE-19 proliferation.Result 1 We successfully cultured ARPE-19 cells, which can fully meet the needs of the study. 2 The immunofluorescence method showed that the expression of S100A7 in ARPE-19 and which located in the cell membrane of experimental groups, but there was no expression in the control group. 3 S100A7 was further confirmed expression in ARPE-19 by western blot technique. 4 MTT was performed to preliminary determine S100A7 promote ARPE-19 cell proliferation.Conclusion Our study is first confirmed the expression of S100A7 in ARPE-19 cells, which can significantly promote ARPE-19 cell proliferation. The study provide a new target and therapeutic direction for human retinal diseases, especially proliferative vitreoretinopathy(PVR) and proliferative diabetic retinopathy( PDR).