| Objective:To confirm the correlation between Kvl.2 expression and the effcet of low-frequency repetitive magnetic stimulation on Aβ25-35-induced murine microglial activation.Materials and Methods : Immunofluorescence and RT-PCR were adopted to observe the Kvl.2 expression of microglia. While Aβ25-35, TsTx and low-frequency repetitive magnetic stimulation were used to stimulate microglia cells, observe the cellular morphology and assay cellular viability by MTT. The expressions of IL-1βmRNA and Kvl.2 mRNA were detected through RT-PCR. ELISA was used to detect IL-1βprotein level. PBFI was treated as a marker to examine the intracellular K+ in cultured cells. The data were analysed by t Test of SPSS13.0.Results:①Microglial viability and morphology were not affected when treated by 20μM Aβ25-35 for 6 hours. Meanwhlie the expressions of IL-1βmRNA and protein were increased, coupled with decreased Kv1.2 mRNA expression.②The concentration of intracellular K+ was increased, to the maximum limit, when treated by 20nM TsTx for 6 hours. In contrast to the effects of 20μM Aβ25-35, the expression of IL-1βmRNA was decreased when 20μM Aβ25-35 and 20nM TsTx costimulated microglia, but the expressions of IL-1βprotein and Kv1.2 mRNA stayed the same level.③The concentration of intracellular K+ was increased, to the maximum limit, when treated by 20 successive pulses of low-frequency repetitive magnetic stimulation, whlie the cellular viability stayed normal. In contrast to the effects of 20μM Aβ25-35, the expressions of IL-1βmRNA and protein were decreased, coupled with increased Kv1.2 mRNA expression.Conclusions:Kv1.2 was involved in Aβ25-35-activated microglia.Coeffect of TsTx and Aβ25-35 could not completely block the activation of microglia, yet low-frequency repetitive magnetic stimulation completely inhibitted its activation by promoting Kv1.2 mRNA expression.
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