| Infertility in married women occupy a very high incidence rate, and become a worldwide difficult cases, infertility is not just a separate disease, but rather an extension of many gynecological diseases. The incidence of infertility in recent years showed a significant upward trend, the study of effective treatment on infertility is significant. Just as in the present, infertility clinic for social work pressure, tension and daily life disorder, diet not quarter caused by deficiency of the kidney, etc, including stomach or spleen stomach proportion of Pixu deficient type infertility, the largest ShuGan subject adopts the spleen method, according to the curative effect of spleen deficiency stomach type infertility breakthrough point, as the curative effect and the different research stack of peony glucoside compatibility of the content of influence.Part One The study on effection of assay of paeoniflorin in Shuganjianpi Prescription and its separate side in different compatibilityObjective:HPLC was applied to determine the assay of paeoniflorin in Shuganjianpi Prescription and its separate side in different compatibility.Methods:High performance liquid chromatography (HPLC) determination of Shuganjianpi Description established parties, such as demolition party compatibility of paeoniflorin in different groups of methodology, that is to adjust the ratio of mobile phase composition, and explore the different components in the determination of paeoniflorin optimum chromatographic conditions. Systematic chromatographic conditions and the following experiment: (1) the standard curve: the preparation of a series of concentrations of paeoniflorin standard solutions, sample test, respectively, were determined by peak area determination, the concentration of paeoniflorin abscissa, the peak area measured for the longitudinal coordinates, the standard curve. (2)Precision: precise amount of paeoniflorin standard solutions, duplication sample 5 times, measured in the chromatographic peak area under the measured precision of the experiment. (3)Repeatability: precise amount of the same set of test solution, the parallel sample 5 times, peak areas were measured to calculate the repeatability of experiments. (4)Stability test: a set of precise amount of sample solution, within 24 hours at regular intervals the sample into the HPLC, the peak area were determined. (5)Recovery test: The determination of sample recovery, taking the known concentration of sample solution were added to a certain amount of paeoniflorin standard reference substance, respectively, of the samples into the sample, the determination of the peak area under the chromatographic conditions to calculate the recovery . And in the choice of the best chromatographic conditions, the peak area measured by external standard in each component of paeoniflorin. To test the peony and spleen drug Atractylodes, Poria, and Liver drug Radix, paeoniflorin Angelica compatibility on the impact of party and its separate Shuganjianpi combinations on each component of paeoniflorin.Results:1 The HPLC methodology established on Shuganjianpi compatibility on whole and split side of paeoniflorin's assay.Adopt consistent with clinical medicine method of preparation of water solution formulation influenza virus. (1) different component of the content of mangiferin paeoniflorin detected by HPLC chromatographic conditions for: chromatographic column for Diamonsic C18 column (5μm, 200×4.6 mm); Ultraviolet detector, detected wavelength: 230nm; Mobile phase: methanol-water (35:65); Velocity: 1.0 ml/min; Column temperature: 25oC; sample size: 10μl; Theory plate number according to the Paeoniflorin peak calculation, should not be less than 2000. In the chromatographic conditions, the measurement of peony glucoside chromatographic peak of retention time 10min, separating degree is about more than 1.5. (2) The liner range for Paeoniflorin was 0.00974～0.05846 mg/ml. Regress equation was Y=12154X+11.435 (R2=0.9997,n=5). (3) Precision measurement of RSD 0.22% (n = 5). (4) the method reproducibility, and RSD is 0.088% (n = 5) .(5) stability test indicated that sample in 24 hours, RSD is stable 0.43% (n = 6) (6) the average recovery of quercetin paeoniflorin 99.47%, RSD is 0.52% (n = 9) that recovery is higher, method are correct and reliable.2 Shuganjianpi compatibility on whole and split side effects of paeoniflorin's assayDetermination of paeoniflorin derived: TGP compatibility with the Bupleurum measured in fractions of paeoniflorin content of 28.48 mg / g, and single herb components measured TGP paeoniflorin 19.49 mg / g compared increased 46.1%; TGP compatibility with Angelica fraction measured content of paeoniflorin 22.96 mg / g, than single herb peony 17.8% increase; peony and Atractylodes Combination fraction measured paeoniflorin was 23.68 mg / g, than single herb peony 21.5% increase; peony and Poria Combination fractions measured the content of paeoniflorin 17.76 mg / g, than single herb peony 8.9% down; Shuganjianpi whole parties measured the assay of paeoniflorin was 25.34 mg / g, than single herb peony 30.0% increase; that TGP compatibility Bupleurum, Angelica, peony after a single taste of peony was significantly higher than improved paeoniflorin in Shuganjianpi whole parties were also significantly increased. Part Two Clinical observation of 60 cases of GanYu PiXu type of infertility with the treatment of Shuganjianpi PrescriptionObjective:Select type of GanYu PiXu 60 cases of clinical cases of infertility, the curative effect on use of Shuganjianpi prescription with the metherd on self-control before and after treatment, and to explore the correlation between paeoniforin's assay and clinical curative effect of Shuganjianpi Prescription.Methods:Collected from 2008 to 2009 hospital expert outpatient service in Hebei TCM hospital, 60 cases of infertility type syndrome belong to GanYu PiXu, including primary infertility and secondary sterility. Shuganjianpi for basic prescription, 2 times a day, and in the morning respectively from 9 to 10 points before sleeping in the evening and take half an hour, every time about 200 ml. With a menstrual cycles for a period of treatment, drug therapy for 3 ~ 6 cycles. Follow the whole concept of traditional Chinese medicine theory, according to patients with specific diseases and appropriate performance on drugs, such as the stomach, add a rose, mint, turmeric and to increase a liver scattered liver clear liver three and effect; The emphasis on the spleen deficiency, add dangshen, yam, GanSong, focal three fairy and to increase the use of spleen filler. Outcome: (1) the clinical symptoms such as observation, the mood irritability fidgety splashed move, and often breast pain, food, stay, and shit is not less, light quality, law tongue or white, thin, white moss pulse condition string or string fine. (2) the menstrual period, observe color, quality, quantity of change. (3) biochemical index in the second day morning menstruation fasting blood, serum sex hormones six level of test. (4) in the B to exceed monitoring menstruation ~ 18 days the 12 B ultrasonic monitoring way, monitoring endometrial thickness and follicular development situation. (5) in the medicine after 1 period on the menstrual cycle delay 10, or more hCG urine test, menopause B ultrasonic detection 5 weeks to germ and original heart beating, determine the palace pregnancy and birth. Live。Results:1 Clinical symptoms in continuous treatment after three periodic 60 patients with menstrual cycle, the law mood stable, in a relaxed mood, food, daily life rhythms, sleep good; But focusing on the Ganyu, symptoms improve significantly better in the spleen deficiency, but mainly is no statistical significance.2 The biochemical treatment after three periodic 55 patients serum sex hormones six levels normal, or close to normal, male hormone estrogen hormone balance coordinate with proportion; Treatment for 6 cycles rest after 5 cases in four patients serum sex hormones six indexes normal coordination, and 1 case PRL is on the high side.3 B ultrasonic testing the treatment of three cycle, 52 patients B ultrasonic detection displayed endometrial and follicular is good, can detect dominant follicular ( diameter follicle≥18 mm) , and endometrial and follicular development size of a periodic variation; Treatment for 6 cycles, except one patient for a side tubal nowhere, intervals of a month can detect advantage follicular; ovulation One patient treatment for 6 cycles, still not detect the advantage follicular outside, the rest B ultrasonic detection, 6 cases that can be detected advantage follicular.4 Conception on taking for Shuganjianpi Prescription treatment in the second period HCG levels for positive, B ultrasonic shows the results of 6 cases; Continuous drug use in the third to four cycles of 29 cases of pregnancy; Continuous drug use in the 5 ~ 6 cycle the conception in 23 cases, the total effective rate was 96%, 2 cases hormone levels tend to be normal, but not conceive.Conclusion:1 High performance liquid chromatography (HPLC) method established by the determination of paeoniflorin in different components of the method: resolution, precision and reproducibility, and can be used to detect different fractions for paeoniflorin.2 Root of herbaceous Peony radix compatibility, angelica, white atractylodes, can raise Shuganjianpi in the spleen party terrace, and content was higher than single herb medicine root of herbaceous peony group. That may be Shuganjianpi Prescription nucleoside herbaceous peony the treatment of Gan Yu Pi Xu ingredient of infertility.3 Shuganjianpi Prescription has some regulatory role in emotional side, very good to improve the patient before treatment such as diet and bowel symptoms.4 On serum sex hormone six parties have significantly improved and coordinating role by Shuganjianpi Prescription, in particular, to reduce PRL, E2 levels increase. And it can promote the development of follicles, the endometrial thickness and follicular development consistent with the size of the trend,which tends to lay the foundation bed.6 Shuganjianpi Prescription has the significantly therapeutic effect on GanYu PiXu type of infertility treatment, and the total effective rate was 96%. And its efficacy correlated with paeoniflorin assay.
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