Merger Nonalcoholic Fatty Liver Disease Of Gallstone Disease Patients CD4~+CD25~+ Regulatory T Cells Changes And Significance

Objective:To test merger nonalcoholic fatty liver disease (NAFLD) of gallstone disease (GD) patients peripheral blood and liver inside of CD4 + CD25 + regulatory T cells (Tregs) level, and the relationship with simple cholelith disease patients, pure nonalcoholic fatty liver disease patients and normal control group of comparison, this paper aims to study in cholelith disease and nonalcoholic fatty liver disease process in vivo sexual TregsT cell changes, for the study of nonalcoholic fatty liver disease pathogenesis of immune and nonalcoholic fatty liver ill patient clinical diagnosis ang treatments with new ideas.Methods:We collected 2009 September - December 2010 treatment in our hospital from the merger of nonalcoholic fatty liver disease of gallstone disease patients who 30 cases, pure cholelith disease patients 40 cases (ALT < 40 U/L), simple nonalcoholic fatty liver disease patients, and choose the same 20 patients in our hospital from the medical examination of normal controls 20 cases. All of the patients without drinking histories and laboratory tests were no viral hepatitis infections history. GD patients all by ultrasound examination and postoperative pathology, hepatic steatosis by ultrasound examination and liver tissue pathology . Nonalcoholic fatty liver diagnostic basis of 2006 liver of the association with alcohol fatty liver learning branch et al "nonalcoholic fatty liver disease diagnosis of guidelines".Application of automatic biochemistry analyzer test serum ALT and liver function, blood fat and blood sugar, etc. And detection the fight - HBV, fight - HAV, fight - HCV, fight - HDV, fight - HEV, fight - HIV, with eosinophilic liver virus infection except other.Take anticlotting peripheral heparin, applicationCD4CD25immune fluorescent antibody, dyeing, erythroid cracking liquid dissolved erythrocyte, completely hemolysis add after 0.02 M phosphat buffer liquid washing, application plus polyphosphate formaldehy - dephosphat buffer liquid fixed, afresh cells suspended, ShangLiuShi cells, lymphocytes detection instrument set door, CD4 + T cells in the analysis of CD4 + CD25 + T cells percentage.HE dyeing observation liver cell pathological changes. A retrovirus polymerase chain reaction monitoring liver tissue mRNA expressions. Within Foxp3.All data were analyzed by SPSS version 13.0 for Windows software. Kruskal-Wallis H test or Mann-Whitney U test was used for comparison between groups. Chi-square test was used for the comparison of constituent ratio between groups. Bilateral(P < 0.05)for difference have statistical sign- ificance.Results:Each common population data and ALT nonalcoholic fatty liver disease patients, body mass index, fasting plasma glucose (FPG), triglycerides (TG), total were higher than those in control group (P < 0.05), the age and gender no obvious difference. Merger nonalcoholic fatty liver disease (NAFLD) of gallstone disease (GD) patients and pure NAFLD patients ALT increased significantly, between the two groups ALT comparison, the difference was not statistically significant (P > 0.05), and the simple cholelith disease patients with normal ALT rcpmaj, difference was statistically signi- ficant (P > 0.05), value are normal control group ALT both in the normal range ( <40U/L).Peripheral blood CD4 + CD25 + T cells level: Merger of NAFLD GD patients (0.049±0.013)and pure NAFLD patients group(0.047±0.015), the pure GD patients(0.036±0.009) CD4 + CD25 + T cells percentage were higher than normal control group(0.025±0.011) (P < 0.05) ,Merger of NAFLD GD patients group and the group of patients with simple NAFLD CD4 + CD25 + Tregs percentage than pure GD patients group (P<0.05). In patients with NAFLD according to the ALT (ALT≥80U/L andALT < 80U/L) are high ALT group and low ALT group, found that high ALT group (0.052±0.015) CD25 + CD4 + Tregs percentage than low ALT group(0.043±0.017) (P < 0.05). In high ALT group NAFLD GD a group of patients (0.057±0.012)with CD4 + CD25 + Tregs percentage higher than a group of patients with NAFLD(0.051±0.008) (P<0.05) . In low ALT patients group NAFLD GD a group of patients (0.042±0.007)with CD4 + CD25 + Tregs percentage compared with a group of patients with NAFLD(0.041±0.013), difference was statistically signifycant (P > 0.05).General pathology and a retrovirus polymerase chain reaction detection with Foxp3 mRNA: Merger of NAFLD GD patients group and pure NAFLD group were conducted liver biopsy, liver tissue in different degree, with central venous fatty change around the most obvious, a big bubble sex, liver cell volume than normal significantly increased, swelling, cytoplasm full of fat vacuoles, nuclei with deviation, fat liver cell boundaries become vague, liver sinus narrow. Merger of nonalcoholic fatty liver disease cholelith disease patients group (0.51±0.12)intrahepatic Foxp3 mRNA expressions of nonal- coholic fatty than pure sex liver ill patient group (0.14±0.08)and pure cholelith disease patients group(0.12±0.07)(P < 0.05), simple nonalcoholic fatty liver ill patient groups and simplicity of cholelithiasis patients group compared not statistically significant difference (P > 0.05), high ALT expressions of group(0.63±0.06)Foxp3 mRNA than low ALT group(0.31±0.05) (P < 0.05).Conclusion:Peripheral blood CD4 + CD25 + T cells level: Merger of NAFLD GD patients and pure NAFLD patients group, the pure GD patients CD4 + CD25 + T cells percentage were higher than normal control group (P < 0.05) ,Merger of NAFLD GD patients group and the group of patients with simple NAFLD CD4 + CD25 + Tregs percentage than pure GD patients group (P < 0.05). In patients with NAFLD according to the ALT (ALT≥80 U/L andALT < 80U/L) are high ALT group and low ALT group, found that high ALT group CD25 + CD4 + Tregs percentage than low ALT group (P < 0.05). In high ALT group NAFLD GD a group of patients with CD4 + CD25 + Tregs percentage higher than a group of patients with NAFLD (P < 0.05) . In low ALT patients group NAFLD GD a group of patients with CD4 + CD25 + Tregs percentage compared with a group of patients with NAFLD, difference was statistically significant (P > 0.05). Show Tregs participation nonalcoholic fatty liver and cholelith disease of the common disease .Tregs with NAFLD patients lier inflammation activities closely related, the progress in NAFLD plays an important role.Merger of nonalcoholic fatty liver disease cholelith disease patients group intrahepatic Foxp3 mRNA expressions of nonalcoholic fatty than pure sex liver ill patient group and pure cholelith disease patients group (P < 0.05), simple nonalcoholic fatty liver ill patient groups and simplicity of cholelithiasis patients group compared not statistically significant difference (P > 0.05), high ALT expressions of group Foxp3 mRNA than low ALT group (P < 0.05). Based on gene expression in the liver Tregs detection, further confir- mation of Tregs and nonalcoholic fatty liver inflamemation sex liver disease patients in closely related, the activities of nonalcoholic fatty liver disease progress plays an important role.