| Objective :IVF-ET pregnancy rate is currently increasing, but there are some patients who have good embryo can not get pregnancy. This cause people pay more attention to implantation defects in infertility patients, the embryo implantation and endometrial development and synchronization with each other is very important, in which endometrial receptivity is a key factor, naturally become a hot research topic. Using suppression subtractive hybridization [1] (suppression subtractive hybridization, SSH) fragments of differentially expressed genes were sequenced, the establishment of endometrial receptivity associated with the differentially expressed gene library screened associated with endometrial receptivity genes, such as galectin-3 and so on. In our study, the analysis and protein galectin-3mRNA pregnancy in IVF group and the previous IVF failure group ovulation cycle secretory phase endometrium to evaluate galectin-3 and the relationship between endometrial receptivity, further exploration of galectin-3 in the reproductive role, to provide the basis to improve the IVF pregnancy rate.Methods:Receptivity from the differentially expressed genes related to library was screened with the genes ofendometrial receptivity in the galectin-3 study options. Choicing the people who take medical treatment at the Fourth Hospital of Hebei Medical University from 2010-1 to 2010-4, all the patients were failed in IVF treatment, due to tubal factor infertility is 9 cases, endometriosis is 2 cases. Control group, 10 cases underwent IVF pregnant women, patients aged 24 to 39 years old, age 2 to 10 years of infertility. All patients were selected subject to the following conditions: preovulatory endometrial thickness equal to or more than 8 mm; form of three lines or close to three lines. Patients collected the previous 6 days after ovulation and the menstrual cycle or 7 days of the endometrium, and stored in liquid nitrogen, part of the tissue fixed with 4% formaldehyde. Application of RT-PCR detection technology, study groups were taken and the control group of about 50 mg of the endometrium to join Rezol 1 ml homogenizer set on fully homogenized, extracted RNA, remove the 0.5μl, was reverse transcription cDNA, then the PCR, both 540 bp amplified fragment length of theβ-actin as internal reference. PCR cycling conditions were: 94℃5 min, 1 cycle; 94℃40 s, 50℃40 s, 72℃50 s, 30 cycles; 72℃10 min, 1 cycle. Products were 1.5% agarose gel electrophoresis, image analysis system with VDS camera and image analysis. IOD values by the reference correction, the correction values for statistical analysis. Immunohistochemical detection: the endometrium fixed with 4% formaldehyde embedded in paraffin, prepared 4μm thick consecutive tissue sections. HE, respectively, and ABC immunohistochemical staining. Dewaxing, digestion, high-pressure antigen retrieval, closed, adding primary antibody (monoclonal mouse anti-human galectin-3 dilution 1:100), secondary antibody, DAB color, hematoxylin, dehydrated and transparent, in the of gum were mounted. Antibody was replaced with PBS as negative control. Microscope and photographed. Positive standard: Each stained slice were randomly selected five vision to clear background color was brown cytoplasm were positive, divided into four levels: positive staining cells did not have any "-"; 25% positive staining cells "+"; 25% to 50% of cells staining positive cells was "++"; more than 50% positive staining "+++".Controlled ovarian hyperstimulation: 6-7 days after ovulation (mid-luteal) to give acetate triptorelin (Ferring Pharmaceuticals, Germany) every 0.1m,down-regulation standards to givegonadotropin (Gonal-F,Switzerland serone company; hMG, Franc pharmaceuticals), the use of transvaginal ultrasound to monitor follicular growth and endometrial changes in the situation,when at least three follicles greater than 20mm in diameter, the evening injection of Azeroth (Switzerland serone company) 250 IU, 38 hours later oocyte recovery, insemination, at D3 after ovulation and embryo transfer, 21 days after transplantation, Type-B ultrasonic was used to confirm clinical pregnancy. All data were used SPSS11.5 statistical software to statistical analysis. Measurement data were±s, differences between groups using t test; count data reported positive rate. The level of statistical significance was set at P<0.05.Results:Comparing IVF failure group and pregnant group',age infertility duration, Gn days, the number of oocytes, the ET number, no significant difference between the number of superior embryos. Galectin-3 mRNA and protein expression in pregnant group is stronger than the non-prengant group. That galectin-3 expression in the study group was 0.11±0.08 (n = 11), increased expression of the control group was 0.42±0.17 (n = 10), P <0.01, statistically significant (p <0.05), galectin-3 protein in glandular epithelial cells and stromal cells were expressed, the expression for the study group was(n=11),"-"1case,"+"7case,"++"表达3case,"+++"表达0case. The control group expressed was(n=10),"-"1case,"+"1case,"++"3case,"+++"5case(p=0.013).Conclusion: Galectin-3 is related to endometrial receptivity one of the genes, the low expression of galectin-3 may cause low endometrial receptivity in IVF failure. The embarrassment of embryo implantion is the foremost reason.
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