| ObjectiveBased on the theory of "You Gu Wu Yun" from "Huangdi Neijing" which is the "bible" for traditional Chinese medicine. Putting the modern scientific research on "You Gu Wu Yun" and "medicine can treat disease cause its toxicity " as the key scientific problem of Chinese medicines toxic appreciation, we proposed that Chinese medicine educe treat effect when they have toxic character. Experimental toxicity studies with safflower as the starting point, with harmacokinetics, high performance liquid chromatography and other modern scientific methods to explore the relationship between drug toxicity and efficacy selective expression and the body's functional state on the theoretical and empirical. Then to confirm that "syndrome pattern"is the decisive factors of selective expression of traditional Chinese medicine toxicity and efficacy. And to further explore the Chinese medicines toxic appreciation.MethodsPregnant mice were divided into 4 groups:control group, blood stasis model group, safflower group and the safflower group, trauma with blood stasis animal model of pregnant mice with a self-made splint in his right leg pressure at the 8-hour mark; with saffron Decoction of saffron group of normal, respectively, to pregnant rats and pregnant rats fed blood stasis in treatment group, control group and model group was given an equal volume of saline. Pregnant rats weighed daily, and observe whether the vaginal bleeding, miscarriage and premature birth; calculate the number of days of pregnancy. Model group recovery or termination of pregnancy when the pregnant rats were sacrificed pregnant mice, abdominal remove the uterus, liver, kidney, and weighing; necropsy uterine horn, the count of live births, stillbirths, birth defects; said the live carcass weight, maternal weight calculation Growth rate, liver (kidney) weight index, and intrauterine growth retardation. Pregnant'mice in each group take the same position right leg quantitative muscle tissue, protein precipitation with methanol processing tissue samples, using HPLC method HSYA A content, chromatographic conditions:C18 column (4.6 mm×250 mm,5μm); detection wavelength:403 nm; mobile phase:acetonitrile-0.2% phosphoric acid (18:82, v/v). ResultsStasis, the safflower group and the safflower group after the termination of pregnancy to reduce fibrinogen (P<0.05), red blood cell accumulation rate decreased, safflower group than the foot swelling to reduce after the model; Aspects of pregnancy and the fetus, compared with the control group, significantly reduced the number of safflower production group (P<0.001), prolonged pregnancy (P<0.05), complete abortion rate of up to 25%,35% unformed embryo, maternal weight gain Slowly (P<0.05), liver weight and kidney weight index were the index increased rate of fetal intrauterine growth retardation (IUGR) up to 100%; and safflower in pregnancy and fetal treatment group, the indicators are close to empty Group. HPLC determination of the uterus in each group and muscle tissue content of HSYA A Show:Red group of muscles 0.1830μg·g-1, safflower group of uterine 0.3409μg·g-1, the treatment group of muscles 0.1068μg·g-1, the treatment group uterus 0.0162μg·g-1.ConclusionSafflower in normal pregnant mice showed toxic effects:reduced production,, prolonged pregnancy, fetal intrauterine growth retardation and even lead to fetal malformation or abortion. The performance of pregnant rats on the stasis model of treatment for the bleeding, pregnancy, toxicity is not obvious:the pregnant rats to reduce foot swelling, decreased fibrinogen, red blood cell accumulation rate. The fetus during pregnancy and had no significant effect on the indicators. HPLC Results:Red Group in the uterus HSYA A was higher than muscle tissue HSYA A content; the uterus of pregnant mice treated HSYA A content of less than Muscle tissue HSYA A concentrations are that the toxicity and efficacy of saffron in the body in different functional role of the state with specific selectivity.
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