The Effect Of Silibinin On Proliferation Of Human Keloids Fibroblasts And The Expression Of MTOR And HIF-1α In Vitro

Objective To investigate the effect of silibinin on proliferation of Human keloid fibroblasts and the expression of mTOR and HIF-1αin hypoxia and normoxia in vitro and explore the mechanism of it.Methods With different concentrations of Silibinin effect on cultured keloid fibroblasts, and CCK-8 assay was adopted to evaluate cell survival. Silibinin and hypoxia were two different intervention factors effect on keloid fibroblasts in vitro. Immunohistochemistry, RT-PCR and western blot were performed to detect the expression of mTOR and HIF-1αbefore and after intervention. Then use statistical software for data processing and statistical analysis.Result Silibinin affect the keloid fibroblasts in different concentrations (25μmol/L,50μmol/L,100μmol/L,200μmol/L,400μmol/L) and different time (24h,48h,72h), we detected the absorbance decreased with the concerntrations and time increased. IHC, RT-PCR and western blot results showed that gene expression and protein expression of mTOR and HIF-1αwere all decreased in 200μmol/L Silibinin concentration in hypoxia and normoxia. There were significant differences between these groups (P<0.05).Conclusions Silibinin significantly inhibited the proliferation of Human Keloids Fibroblasts cell in vitro in a concentration and time dependent manner. Silibinin also inhibited the expression of mTOR and HIF-1αin hypoxia and normoxia.