Gene ACE2 Inhibited The Expression Of Angiotensinⅱ Type 1 Receptor MRNA Induced By Angiotensinⅱ In VSMCs Of SHR

AIM:To investigate the effects of gene ACE2 transfection on expression of AngⅡtype 1 receptor mRNA in vascular smooth muscle cells of SHR. METHODS:Transfect pm-ACE2 into vascular smooth muscle cells of SHR by lipofectamine 2000. VSMC were devided into normal cell group,pcDNA3.1/Hygro(+) transfected group,pm-ACE2 transfected group,AngⅡgroup,pm-ACE2 transfected + AngⅡgroup. Detecting the expression of AngⅡtype 1 receptor mRNA through using RT-PCR.RESULT: The OD value of AngⅡtype 1 receptor mRNA of ACE2 transfected group were obviously lower than that of normal cell group and pcDNA3.1/Hygro(+) transfected group, the effect was time dependence (12h—72h)(P<0.05)and concentration dependence(4ug—10ug)(P<0.05). The effect of AngⅡon expression of AngⅡtype 1 receptor mRNA was concentration dependence .When the concentration of AngⅡwas 10-8mol/L ,the expression of AngⅡtype 1 receptor mRNA was higher than that of normal cell group or pcDNA3.1/Hygro(+) transfected group. The OD value of pm-ACE2 transfected + AngⅡgroup was obviously lower than that of normal cell group and pcDNA3.1/Hygro(+) transfected group(P<0.05),but obviously higher than that of pm-ACE2 transfected group.CONCLUTION:The expression of AngⅡtype 1 receptor mRNA can be inhibited through transfecting pm-ACE2 into vascular smooth muscle cells of SHR, the effect was time dependence (12h—72h)and concentration dependence(4ug—10ug). ACE2 can reverse the effect of AngⅡ.