Proliferation Inhibitory Effect Of Optimal Combination Of Ginsenoside Rg3, Curcumin And Quercetinon On Bladder Cancer Cell

Objective bladder cancer is a commonly occurring cancer in urological system. The current therapies for transitional carcinoma cell (TCC) of the bladder include local resection, chemotherapy, radiotherapy and other methods. But the effect is not yet satisfactory. Explore novel therapies are necessary. Rich resources of Chinese medicine for anti-tumor provide new ideas for studies. This study is designed to confirm the growth inhibition effects of the optimal combination of Ginsenoside Rg3, Curcumin and Quercetinon which is obtained by orthogonal experiment through Pre-test in human bladder cancer cells and to explore its possible anti-tumor mechanism.Methods Pre-optimized orthogonal test obtained the Optimal Combination of Ginsenoside Rg3, Curcumin and Quercetinon as ginsenoside Rg3 160μmol/L, quercetin 38μmol/L, curcumin 24μmol/L. Human bladder cancer cell lines BIU-87, EJ are treated with the optimal combination of Ginsenoside Rg3, Curcumin and Quercetinon for 24 hours. Then, cells proliferation were measured by tetrazolium salt (MTT), At the same time carried out the colony formation assay. Cell apoptosis and changes in cell cycle were examined by Flow cytometry(FCM) on BIU-87 cells. DNA injury of human bladder cancer cells EJ and BIU-87 were assessed by Single cell gel electrophoresis assay. RB protein expression was detected by western blot.Results (1) MTT test confirmed that optimal combination of Ginsenoside Rg3, Curcumin and Quercetinon significantly inhibited the proliferation of human bladder cancer cells, compared with the single drug component groups, cell growth inhibition rate of optimal combination of Ginsenoside Rg3, Curcumin and Quercetinon was statistically different (P<0.05).The combination treated BIU-87, EJ bladder cancer cell for 24 hours, cell growth inhibition rate reached 52.7% and 55.3% respectively.(2) after treatment of the combination, cloning efficiency of BIU-87 and EJ cells were 2.85% and 3.65% respectively.(3) combination treatment caused BIU-87 cells arrest at GO/G1 phase, and S phase cells decreased.(4) DNA damage of human bladder cancer cells BIU-87 and EJ caused by combination compared with the single drug component has significant difference (P<0.05).(5) western blot results showed that there was no effects on the expression of Rb protein in BIU-87 cells.Conclusion Optimal combination of Ginsenoside Rg3, Curcumin and Quercetinon is able to inhibit human bladder cancer cell lines growth in vitro, arrest cell cyle at GO/G1 phase, and lead to apoptosis. The optimal combination of Ginsenoside Rg3, Curcumin and Quercetinon has siginificant DNA damage effects. Optimal combination of Ginsenoside Rg3, Curcumin and Quercetinon may be a potential chemotherapeutic drug for bladder cancer.