Study On Biotransformation Of 4-phenylcoumarin By Two Cultured Plant Systems

The paper studied on the glycosylation of 4-phenylcoumarin by hairy root of Polygonum multiflorum Thunb. and cell suspension cultures of Catharanthus roseus so as to build a high efficient and selective method of glycosylating coumarins.To investigate the regioselectiveity of glycosylation, two coumarin were synthesized by the Pechmann reaction:7-hydroxy-4-phenylcoumarin and 5,7-dihydroxy-4-phenyl-coumarin. In their glycosylation researches, tow compounds were produced by hairy root of Polygonum multiflorum and cell suspension cultures of Catharanthus roseus. Transformed products were detected by TLC and HPLC, which were isolated by silica-gel column chromatography. The structures were elucidated by 1H-NMR, 13C-NMR, HMBC and ESI-MS spectral analysis. The structure of product was determined as 4-phenylcoumarin-7-O-β-D-glucopyranoside (BCP1) and 7-hydroxy-4-phenylcoumarin-5-O-β-D-glucopyranoside (BCP2). The later was a new compound. Conversion conditons of BC01 and BC02 were optimized by different co-culture time. Time-course curve showed the best co-culture time of BC01 and BC02.