Studies On The Chemical Constituents Of The N-BuOH Extract Of Paris Bashanensis

To search after the alternative resource for the Chinese traditional medicine Rhizoma Paridis, the chemical constituents of Paris bashanensis, which belongs to the same genus as Paris polyphylla var.chenensis and P. polyphylla var. yunnanensis, the traditional resource of Rhizoma Paridis, were studied. The ethanol extract of rhizome of P. bashanensis was divided according to polarity into petroleum ether extract, ethyl acetate extract, n-BuOH extract and water soluble extract. The n-BuOH extract of P. bashanensis was applied to silica gel column, reverse phase silica gel column, Sephadex LH-20 column and Pre-RP-HPLC in turn to give five pure compounds. On the basis of chemical reaction an data analysis of their spectral data of MS,1H-NMR,13C-NMR,1H-1H COSY, HSQC and HMBC, the structures of the five compounds were elucidated asβ-ecdysone (1), pinnatasterone (2), pennogenin-3-O-α-L-rhamnopyranosyl(1→2)-[α-L-arabino furanosyl(1→4)]-β-D-glycopyranoside (3), diosgenin-3-O-α-L-rhamnopyranosyl (1→2)-[α-L-arabinofuranosyl(1→4)]-β-D-glycopyranoside (4), pennogenin-3-O-α-L-rhamnopyranosyl(1→4)-α-L-rhamnopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-gly copy-ranoside (5). The last three steroid saponins have been reported existing in the Chinese traditional medicine Rhizoma Paridis. The similarity of the chemical constituents of P. bashanensis and Rhizoma Paridis suggests that P. bashanensis may be an alternative resource of Rhizoma Paridis.