Objective:Through the experimental merhods of MTT,Flow Cytmetry,Western Blotting to detect micromolecule compound S1 have an influence on mice melanoma B16 cell apoptosis ,to explore the mechanism of S1 induce apoptosis,to provide determinate deriction for enhancing S1 tumor inhibition .Method: After B16 cell to give S1,MTT to detect cell increment rate, Flow Cytmetry to detectapoptosis rate, Western Botting to detect the change of the expression level of endoplasmic reticulum and mitochondria apoptosis associated protein.Results :The result of MTT display,S1 might obviously decrease B16 cell increment rate ,the difference has statistical significance(P<0.05).The result of Flow Cytmetry display ,S1 might induce obviously increased of B16 cell apoptosis rate ;while the result of Western Blotting display ,apoptosis associated molecule activated the expression level of caspase-3 obviously increase , the difference has statistical significance(P<0.05). The result of Western Blotting display, the expression level of endoplasmic reticulum stress associated protein GRP78 and endoplasmic reticulum stress-apoptosis associated protein CHOP obviously increase , the difference has statistical significance(P<0.05);while the expression level of mitochondria apoptosis associated protein Cytchrome C also occurrence obviously increase ,the difference has statistical significance(P<0.05).Conclusions :1. Micromolecule compound S1 might obviously inhibit cell increment rate .2. Micromolecule compound S1 might cause B16 cell to occurrence obviously apoptosis.3. Micromolecule compound S1 possibily cause B16 cell to occurrence apoptosis through endoplasmic reticulum stress and mitochondria apoptosis.
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