PEGFP/Ang-1 Transfected Rat BMSCs Transplantation On Diabetic Retinal Vascular Leakage Of Retinal

ObjectiveUse of Pichia pastoris recombinant angiopoietin 1 (pEGFP/Ang-1) transfected with rat BMSCs, BMSCs transfected lines after subretinal transplantation, thus confirming the angiotensin 1 (Ang-1) transfected BMSCs on diabetes Retina in rats. Use of Pichia pastoris recombinant angiopoietin 1 (pEGFP/Ang-1) transfected with rat BMSCs, after transfection Ang-1/BMSC the subretinal transplantation.MethodsMale SD rats were selected from 4 to 6, under sterile conditions remove the femur and tibia, cut epiphyseal end of the bone marrow cavity with repeated washing medium isolated BMSCs, combined with repeated adherence to further purification and identification. Replacement of the 96 SD rats were randomly divided into normal rats (CON), diabetic control group (DM1), subretinal transplantation in diabetic rats + BMSCs group (DM2), subretinal transplantation in diabetic rats + Ang-1 / BMSCs group (ANG). Intravenous injection of streptozotocin (STZ) model of established diabetes, DM2 group after modeling subretinal transplantation BMSCs, ANG Group subretinal transplantation Ang-1/BMSCs, respectively, 4w, 8w, 12w animals were sacrificed..Detected by Evans blue infiltration capacity of the retina. Immunohistochemistry using transplanted cells in the retina of the distribution of fusion. Western blot method to detect the retinal expression of Ang-1.ResultsDM1, DM2 and diabetic rats with a large amount of infiltration and permeability increased gradually with time; SIM group subretinal transplantation Ang-1/BMSCs can inhibit this change. Immunohistochemical analysis confirmed that the main integration Ang-1/BMSCs diabetic rats transplanted retinal ganglion cell layer and internal granular layer. Western blot analysis showed that with the development of diabetic retinopathy, STZ-induced DM1, DM2 and diabetic rat retina the expression of Ang-1 significantly reduced, while the ANG group, Ang-1 expression was significantly increased, and DM1, DM2 There was significant difference between groups (P <0.05), DM1 and DM2 group were significantly (P> 0.05).Conclusions1,Ang-1/BMSCs retinal integration of transplanted cells after transplantation see in the ganglion cell layer, inner granular layer.2,Ang-1/BMSCs subretinal transplantation of retinal microcirculation of diabetic rats.