Correlation Between Polymorphism Of Interleukin-13 Gene And Susceptibility To Chronic Obstructive Pulmonary Disease In Xinjiang Uyger Population

Objective:To study the association between polymorphism of IL-13 gene promoter-1055C/T, exon+2044A/G, intron+1923C/T and susceptibility to chronic obstructive pulmonary disease in Xinjiang Uyger population. Methods:220 stable COPD patients (the case group) and healthy controls (the control group) of 220 age-and-sex matched with the case group, unrelated individuals were recruited in a case-control study to the Uygur of Hetian region. The venous blood and the check index were collected. Whole blood genomic DNA were extracted, DNA fragment was amplified by Polymerase chain reaction (PCR), DNA sequencing were identified by single nucleotide polymorphism (SNPs) cation. Data were analysised by SPSS 17.0 software. LD and haplotype were analysised by SHEsis software. Results:(1) For IL-13 gene promoter-1055C/T locus, comparison of genotype frequency distribution of CC, CT and TT isχ2=14.185 and P<0.05 in case group and control groups; Comparison of allele frequency distribution of C and T isχ2=16.055, P＜0.05; In Logistic regression analysis, OR of CT genotype vs CC genotype is 2.155(95%CI:1.286-3.614), OR of TT genotypes vs CC genotype is 2.705(95%CI:1.103-6.634). (2) For IL-13 exon+2044G/A locus, comparison of genotype frequency distribution of GG, GA and AA isχ2=1.518 and P>0.05 in case group and control groups; Comparison of allele frequency distribution of G and A isχ2=0.880 and P >0.05. (3) For IL-13 gene intron+1923C/T locus, comparison of genotype frequency distribution of CC, CT and TT isχ2=3.191and P>0.05 in case group and control groups; Comparison of allele frequency distribution of C and T isχ2=1.410 and P>0.05. (4) Pairwise LD analysis found there were no linkage disequilibrium in three SNPs. (5) Three SNPs loci constitute 8 haplotypes, and three haplotypes frequency distribution in the case group and control group were significantly different. Conclusion:The genetic polymorphism in promoter-1055C/T locus of IL-13 was associated with the susceptibility to COPD in Xinjiang Uyger population. The genetic polymorphism in Exon +2044G/A locus of IL-13 was not associated with the susceptibility to COPD in Xinjiang Uyger population. The genetic polymorphism in Intron+1923C/T locus of IL-13 was not associated with the susceptibility to COPD in Xinjiang Uyger population. There were no linkage disequilibrium in three SNPs. Three haplotypes may be associated with COPD in Xinjiang Uygur population.