The Relativity Study Of The Mitochondrial Gene Mutation Of Non-syndromic Hearing Loss And Kidney Deficiency With Blood Stasis Type Of Uigur And Han

Objective:To study the relative of Kidney asthenia blood stasis type deafness and mtDNA 12srRNA gene mutation, the normal people and sensorineural hearing loss in patients in the Uyghur nationality, and compare the differences between the two groups; To study the relevant between mutationof deafness with kidney deficiency and blood stasis with mtDNA12S rRNA。Methods:137 deaf patients and other 93 normal person had been gathered,137 cases which tinnitus and deafness with differentiation of symptoms and signs for classification of syndrome, divided into kidney asthenia blood stasis (105 cases) and non-blood stasis Kidney and Blood(32 cases).to obtained the blood from the 230 samples people,the mtDNA 12srRNA were examined by polymerase chain reaction(PCR), and statistics analysis the relative between Kidney asthenia blood stasis type deafness and mtDNA12srRNA. Result:Many types the mutation had been detect of mtDNA12srRNA and the mutation ratio of A1555G was 4/105(3.8%) higher in patients with hearing loss than the average. The disease-causing mutations T961cn,A827G,nt955ccccnt960 were detected, All of them were not be deteced in the normal person. Many types polymorphism the mutation had been detect of mtDNA12srRNA:G709A,A750G,A1438G and they bear out the mtDNA mutation polymorphic.lt was also found a new case of mutant forms:nt950cccccnt956, The pot nt950cccccnt956 may be the new disease-causing mutations type.The differences between Hearing loss group and by mutations in the control group were statistically analyzed, The results showed that the difference was statistically significant,(P<0.05),There is statistically significant differences between Kidney asthenia blood stasis type and mtDNA 12S rRNA gene mutation, There was significant difference (P<0.05),that is Kidney asthenia blood stasis type in Blood Uygur has the relationship with mtDNA 12S rRNA gene mutations.Conclusion:There were mutually relative between the type of kidney asthenia blood stasis and mtDNA 12srRNA gene mutation in Uyghur patients with hearing impairment in xinjiang. Kidney asthenia blood stasis type might be the reason of mtDNA 12srRNA gene mutation in the Xinjiang region, and there was certain ethnic and geographical characteristics. that carry mutations in the gene for deaf individuals with disease susceptibility, so scientific intervention will help reduce or delay the hearing loss diseases.