Immunofluorescence Study Of LPS-induced NF-κB Expression In Human Decidual Cells

Objective:The aims of this study were to investigate the activation of nuclear factor kappa B (NF-KB)-induced by various doses of Lipopolysaccharide (LPS) on first trimester human decidual cells. Method:Human decidual cells were obtained from 10 normal early pregnant women who were performed induced abortion. Decidual cells were stimulated by various concentrations of Lipopolysaccharide (LPS) and NF-kB activity was assessed by Immunofluorescence staining method Results:LPS induced inflammatory response by translocation and activation of NF-KB. There was statistical significance when compared each group and reference value (P≤0.05). All calculations were performed using SPSS V 13.0 for Windows.Data are presented as mean±SD. Comparisons were made using a one-way ANOVA Dunnett's T3 test. Conclusion:This study suggest that LPS is a signalling pathway involved in activation of NF-kB in primary human decidual cells and consistently confirm that decidual cell is an important site of NF-kB regulation.