Expression Of COX-2 And Hif-1α, And Its Correlation With Microvascular Angiogenesis In Cervical Cancer

Objective:Discuss the effect and clinical significance of COX-2 and HIF-1αin the development of cervical carcinoma histopathology; Research the relationship between the expression of COX-2 and HIF-1αwith the angiogenesis in cervical cancer through analysising the relevance between the expression of COX-2,HIF-1αand micmvascular density (MVD),which marked by human hematopoietic progenitor cell antigen (CD34) ;In order to provide a new reference indicators for the early diagnosis and treatment of cervical cancer.Methods:Detectin the expressin of COX-2 and HIF-1αin normal cervical epithelium,cervical intraepithelial neoplasia and cervical cancer tissue by using tissue slice and immunohistochemistry;Analysis the relationship between the expression of COX-2 and HIF-1αwith the histological type,histological grade ,clinical stage of cervical cancer by the statistical software SPSS13.0, also analyzed the correlation with MVD which marked by CD34. Results:1,The Positive rate of COX-2 ware 5.0%,45.2%,89.3% in the histological type,histological grade and clinical stage of cervical cancer, there ware significant differences between them(P < 0.05); The Positive rate of COX-2 ware 72.7%,86.8%,100% in well-differentiated,differentiated and poorly differentiated cervical cancer, there ware significant differences between them also (P<0.05)。2,The positive rate of HIF-1αware 0%,45.2%,80.6% in normal cervical epithelium, cervical intraepithelial neoplasia and cervical cancer tissue, there ware significant differences between them(P < 0.05); The positive rate of HIF-1αware 50%,76.3%,100% in well-differentiated,differentiated and poorly differentiated cervical cancer, there ware significant differences between them also (P<0.05);。3,The range of the MVD in 165 case normal cervical epithelium, cervical intraepithelial neoplasia and cervical cancer tissue was 7.6~82.2,the median was 50.8(51.5±7.7).It showed correlation between the MVD and the expression of COX-2,HIF-1αand CD34 in the three types organizations.Conclusions:1,The expression of COX-2 and HIF-1αhad relation with tumor differentiation and metastasis in cervical cancer.2,It indicated that COX-2 and HIF-1αparticipated the microvessel formation in cervical cancer because there had correlation betweem COX-2,HIF-1αwith MVD which marked by CD34.