| Objective: CHD5 (chromodomain helicase DNA-binding 5) gene was deleted, mutated and methylated in a variety of tumors, including cervical cancer. CHD5 play an important role in suppressing the development of cancer. Studies shows that CHD5 gene can regulate proliferation, senescence and apoptosis by p19arf/p53, p16INK4a/pRb pathway in cell. To further understand the function of CHD5 in cervical cancer, CHD5 expression level in cervical issue was detected by immunohistochemisty(IHC) and explored the function of CHD5 on proliferation and cell cycle in HeLa cells after down expressing CHD5 using RNAi by MTT and flow cytometry.Methods: 1. The expression level of CHD5 was detected in cervical cancer and normal cervical tissue by IHC. 2. Recombinant adenovirus vector with CHD5 siRNA was constructed and infected HeLa cells . Expression of CHD5 was detected by RT-PCR and western blot after infection. 3. The grow and cell cycle distribution of HeLa cells were detected by MTT and flow cytometry respectively after infection.Results: 1.IHC detection showed that the expression of CHD5 was lower in cervical cancer compared with normal cervical tissue (P<0.05). 2.The recombinant CHD5 siRNA adenovirus were constructed correctly with a titer of 5×1011pfu/ml. Ad-CHD5siRNA inhibiting the expression of CHD5 was detected in HaLa cells after infecting. 3. MTT results demonstrated Ad-CHD5siRNA promoted the grow of HeLa cells. Flow cytometry indicated the cell cycle distribution of HeLa cells was changed after infecting with Ad-CHD5siRNA. HeLa cells on G0/G1 phase were reduce while in S phase were increase. Cells numbers on G2/M phase were not changed.Conclusion: 1. The expression level of CHD5 in cervical cancer is lower than in normal cervical tissue. 2. The recombinant CHD5 siRNA adenovirus were abtained. 3. CHD5siRNA adenovirus infection results suggest that HeLa cells may go through the G1 / S checkpoint into S phase more easily, promote proliferation when the expression of CHD5 reduced.
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