Effect Of Propofol On Synapic Long-Term Potentiation Of Hippocampal Slices Of Rats With Vitamin A Deficency

Objective : In vitro hippocampal slices as experimental object, adopting extracellular recording technique, to observe the effect of propofol on long-term potentiation(LTP) of hippocampal slices of rats with vitamin A deficiency.Method:Select rats randomly from the normal rats and rats which lack of vitamin A, kill the rats for preparation of hippocampal slices, mix gases to saturate in artificially cerebrospinal fluid (aCSF)for 2 hours, and then select better ones into the experiment. Brain slices divide into four groups:N1, normal control group, not lack of vitamin A, not add propofol.N2,normal group, not lack of vitamin A, add propofol into Cerebral perfusion fluid to final concentration 50μmol/L.VAD1,VAD control group, lack of vitamin A, not add propofol.VAD2,lack of vitamin A, add propofol into Cerebral perfusion fluid to final concentration 50μmol/L.Frist, evoke population spike (PS) in single channel impulse stimulation (test stimulation, wave wide 0.2 ms), every 5 min records a PS amplitude, take the average of 3 times as the baseline.Then change the perfusion fluid of N2 and VAD2 to propofol of 50μmol/L (velocity:1ml/min),then give single channel impulse stimulation(strength and wave wide same as test stimulation), once every 10minutes, observate for 1 hour. Then washed out propofol with artificially cerebrospinal fluid (aCSF) , give 100HZ high-frequency stimulation (stimulation intensity and wave same as test stimulate), take a 10 minutes rest,and give single channel impulse stimulation, once every 10 minutes , observate for 1 hour. N1 and VAD1 did not add propofol, but give the same high-frequency stimulation (HFS) and get PS record, observate for 1 hour.ResultAfter HFS,Compared to N1, LTP relative amplitude of N2 was obviously inhibit,much lower than N1. Compared to VAD1, LTP relative amplitude of VAD2 was obviously inhibit,much lower than N1. Compared to N2, LTP relative amplitude of VAD2 get lower, and its PS amplitude almost had no rise.ConclusionPropofol can inhibit LTP of hippocampal slices obviously, and to a rat of vitamin A deficiency,the degree of inhibit is deeper than nomal one.