| Objective To compare the promotion of three different motor training paradigms in rats after spinal cord injury(SCI).Method 45 female adult Sprague-Dawley rats were used. An animal model of incomplete spinal cord injury was created using a modification of Allen's method at T9 spinal cord. Taking the uninjured rats as the normal group, and the injured rats without training as the control group. Three groups of injured rats were trained by different motor training paradigms , body-weight-support-treadmill- training(BWSTT), swimming training and wheel running. Exercise training began at the 8th day post surgery, lasted 30 minutes per day, 5 days a week for 4 weeks. Locomotor functioning was evaluated using inclined plane tests,modified Tarlov scores and Basso—Beattie—Bresnahan(BBB)scales before the operation and on the 1st day, and the 7th, 14th, 21st, 28th, 35th day post surgery.Histomorphological changes of T9 spinal cord and gastrocnemius muscle were observed by light microscope and electronmicroscope , and the cross section area and diameter of the gastrocnemius muscle fibers were analyzed by specialty computer software.Results①The angles of the inclined plane test ,the Tarlov scores and the BBB scores of BWSTT group and swimming training group had increased significantly compared with the control group at any time of the observation(P<0.05), and it was no significant difference between the two groups(P>0.05).Although the scores of the wheel running group had increased, it was no significant difference compared with the control group(P>0.05).②The observation through light microscope and electronmicroscope of the T9 spinal cord of the three training groups showed that the improvement of the BWSTT group was the most significant. After 4 weeks of BWSTT ,the edema of the injured T9 spinal cord tissue has decreased ,cell vacuolar degeneration was lessen, the shape of neuron and glial cell was partly recovery, and the nerve fiber hyperplasia was significant.③Compared the shape , the cross section area and the diameter of the gastrocnemius muscle fibers of three training groups, it showed that the improvement of amyotrophy of BWSTT group was the most significant. Its cross section area and diameter was closed to the normal group .The swimming training group also has great improvement compared with the control group, while the wheel running group was not significant.④From the perspective of the tolerance of training and the security, the BWSTT group and the wheel running group were more safety. The swimming group had safety risk, for there were 2 injured rats drowned while training.Conclusions The three motor training paradigms can improve the motor activity and the nerve- muscle function at different extent, and the effect of the BWSTT group and the swimming training group was better than the wheel running group.Considering the tolerance and the security of training,we think the BWSTT is a more compatible motor training paradigm for spinal cord injury rat. Objective To explore the promotion of body-weight-support-treadmill training (BWSTT) and the changes of secondary lesion in distal spinal motor neuron in rats after spinal cord injury(SCI).Method 30 female adult Sprague-Dawley rats were used. An animal model of incomplete spinal cord injury was created using a modification of Allen's method at T9 spinal cord. Taking the uninjured rats as the normal group, and the injured rats without training as the control group. One group of injured rats were trained by BWSTT. Exercise training began at the 8th day post surgery, lasted 30 min per day, 5 days a week for 4 weeks. Locomotor functioning was evaluated using inclined plane tests, modified Tarlov scores and Basso—Beattie—Bresnahan(BBB)scales before the operation and on the 1st day, and the 7th, 14th, 21st, 28th, 35th day post surgery.Histomorphological changes of L5 spinal cord and gastrocnemius muscle were observed by light microscope and electronmicroscope , and the cross section area and diameter of the gastrocnemius muscle fibers were analyzed by specialty computer software.Results①The angles of the inclined plane test ,the Tarlov scores and the BBB scores of BWSTT group had increased significantly compared with the control group at any time of the observation(P<0.05).②Compared the shape, and the cross section area and the diameter of the gastrocnemius muscle fibers, it showed that the improvement of amyotrophy of BWSTT group was significant. Its cross section area and diameter was nearly closed to the normal group (P>0.05).③The observation through light microscope and electronmicroscope of the L5 spinal cord of each group showed that the improvement of the BWSTT group was significant. After 4 weeks of BWSTT, neurites and Nissl bodies didn't have great decreased, the shape of myelin sheath and neuraxis was mostly integrity, and the vacuole in the cell matrix was significantly decreased.Conclusions The body-weight-support-treadmill training can significantly improve the motor activity of spinal cord injury rats , and effectively decrease the secondary lesion in distal spinal motor neuron.
|
PDF Full Text Request