The Mechanism Of AIF On Rat Pulmonary Fibroblasts Apoptosis Induced By Curcumin

Objective In order to explore the molecular mechanism of apoptosis-inducing factor (AIF) on rat pulmonary fibroblasts, investigating the proliferation and apoptosis effect of curcumin.Methods With curcumin/ Z-DEVD-fmk -stimulated cells for the control group, fibroblasts derived from lung tissue of rat were cultured in vitro. Cultured cells were exposed to curcumin with different final concentration (5μM,10μM,20μM,40μM) and Z-DEVD-fmk with final concentration(20μM). MTT assay was used to determine the inhibitory rate of curcumin on the proliferation of cells. Flow cytometry identified the apoptosis. Western-blot was used to determine the expression and nuclear translocation of AIF protein.Results Curcumin inhibited proliferation of pulmonary fibroblasts in a dose- dependent manner (5μM,10μM,20μM,40μM),which was observed Flow cytometry. But the proliferation inhibitory and the apoptosis induced by curcumin were not inhibited by Z-DEVD-fmk.In curcumin treated groups, AIF was observed associated with the nuclear translocation by Western-blot.Conclusion Curcumin inhibited proliferation of pulmonary fibroblasts. The curcumin-induced apoptosis may be associated with the releasing of AIF from mitochondria.