The Application Of High-risk HPV Testing In Huierarchicl Management Of Patients With Atypical Squamous Cells Of Undetermined Significance

ObjectiveTo evaluate high-risk HPV test in hierarchical management of women with atypical squamous cells of undetermined significance (ASCUS) by using HC-Ⅱ。Methods686 cases of cervical cytology result of ASCUS were treated with the Hybrid Capture test (HC-2) sampler to collect specimens, using immunological techniques and by chemical luminescence microplate signal amplification to gene in samples of viruses on trace detection. The 13 most common high-risk HPV detection, diagnosis criteria for the positive specimen detected HPV DNA was greater than or equal to 1.0pg/ml. Also observed in this group of patients of all ASCUS colposcopy and cervical biopsy examination of endoscopic biopsy of suspicious lesions. If no obvious cases of abnormal or images were not discovered in the microscope, you got more points of cervical biopsy or endocervical curettage surgery to check. All biopsy specimens were sent to department of pathology, the pathologist who read the piece and made the final diagnosis, the pathological diagnosis as the gold standard. 402 of them in patients with ASCUS cervical cytology and HPV test for high-risk follow-up, were carried out during follow-up cervical cytology, high-risk HPV test and colposcopy, if necessary, the multi-point cervical scraping biopsy or neck surgery sent to histopathology. Results1. ASCUS category in all poor reproducibility, and its pathological diagnosis of biopsy results have diversity.2. As the severity increased and higher pathological grade, high-risk HPV infection rates increased significantly.3. In patients with ASCUS, HPV-positive HSIL detection rate was significantly higher than the negative group, statistically significant difference test (p <0.05). High-risk HPV negative cervical carcinoma was no place.4. The pathological findings as the gold standard to calculate the detection of high risk HPV negative predictive value is high.Conclusions1. ASCUS classification is repetitive TBS worst of all, colposcopic biopsy diagnosis of pathology is diverse, hierarchical management for patients with ASCUS is necessary.2. High-risk HPV infection and cervical cancer and cervical precancerous lesions was closely related to the severity increases with the increase of pathological grade and high-risk type of HPV infection increased significantly, high-risk HPV negative predictive value was high, the application of patients in the ASCUS High-risk HPV test for hierarchical management of great significance.