The Correlative Research Of The Tissue Microenvironment And COX-2 Expression Of T-cell Lymphoma To Chemotherapy Effect

Background & Objective: T-cell lymphoma has the poor efficacy through conventional chemotherapy, the pathological of tumor microenvironment is characterized by inflammation within the significant infiltration of inflammatory cells and the presence of a variety of inflammatory cytokines; It is not clearly that tumor tolerance to chemotherapy in related to inflammatory cells and inflammatory cytokines.This study detects the quantity of macrophages and the expression of macrophage inflammatory cytokines which is in the microenviroment of tumor tissue from the patients untreatment, and combined with the efficacy evaluation with CHOP regimen and long-term survival to analyse their correlation through statistical methods. To investigate COX-2 expression was correlated with macrophages content and inflammatory cytokines how to affect the chemotherapy effect by detecting the expression of COX-2 in the tumors cells. Analysis the relationship of the clinical features of T-cell lymphoma and macrophages content and COX-2 expression, observe the relationship of the clinical features of T-cell lymphoma and prognostic. These results of the surveys have a certain value on the impact of chemotherapy and to find new ways to improve the efficacy of chemotherapy by exploring the microenvironment of T-cell lymphoma.Methods: To study 45 cases TCL in patient of clear pathological, tumor tissue sample, first chemotherapy with CHOP regimen and clear follow-up data. Application of CD68 monoclonal antibody on specimens by immunohistochemistry (IHC) markers to calculate the number of CD68 positive cells in the samples. And to detect the inflammatory cytokines IL-6, IL-8 protein expression come from the macrophages and COX-2 protein expression of tumor cell in the tissue samples by IHC. The data was analyzed by SPSS13.0 Statistical package.Results: The normal group of macrophages content more effective than the increased on chemotherapy effect (ORR 88.8% compared with 44.4%, P = 0.008; CR 33.3% compared with 5.6%, P=0.028), the difference was statistically significant. IL-6, IL-8 expression were not related with the curative effect of chemotherapy. The group of COX-2 negative expression more effective than COX-2 positive expression on chemotherapy effect (ORR94.1% compared with 57.1%, P=0.010; CR35.3% compared with 14.3%, P=0.100). The relationship of COX-2 expression and macrophages content show a positive correlation (r=0.356 P=0.017), but was not correlation with IL-6 and IL-8. There is no relationship of macrophages content and age, gender, B symptom, the number of extranodal involvement organs, the level of LDH and Ann Arbor stage, besides Short-term chemotherapy effect, PS score, prognostic index score, with or without bone marrow involvement. except the Ann Arbor stage and marrow invasion, COX-2 has no relationship with any other clinical features. Except the gender, age, B symptom, Short-term chemotherapy effect, IL-6 and IL-8, the long-time survival is related to Ann Arbor stage, the level LDH, PS score, prognosis index scores, with or without marrow invasion, the number of extranodal involvement organs, macrophages content and COX-2 expression.Conclusions: It is a negative correlation that the between the high macrophages content and the high expression of COX-2 and the chemotherapy effect and long-term survival about T-NHL. The expression of IL-6 and IL-8 have nothing to do with chemotherapy effect and long-time survival. The relationship of COX-2 expression and macrophages content show a positive correlation (r=0.356 P=0.017), but was not correlation with IL-6 and IL-8. The independent factors of affecting survival were Clinical stage, LDH level and bone marrow involvement.