| Objective: To document changes of slow-wave (slow wave) and observe changes of the number, structure and morphology in interstitial cells of Cajal when disorder occurred in motility of duodenum in diabetic mice .Methods: Male balb / c mice were divided into normal control group (Control group), diabetic group (DM group). DM model was established by intraperitoneal injection of streptozotocin(STZ, 150 mg/kg,ip)in Balb/c male mice ,and phosphat buffer solution (pH = 7.40)was given in Control group. All the mice, after 6 weeks, intervention, were given Indian ink by lavage for the small intestinal transit rate determination. Finally execute all the mice, slow waves of duodenal smooth muscle cells were recorded by using intracellular microelectrode recording instrument. Observe changes of ultrastructure in ICC by transmission electron microscopy .Results: Small intestine transit rate of DM group was significantly reduced than that of the Control group (44.05±5.48 VS 82.75±6.56, P<0.01); Compared with normal mice, slow wave frequency of intestinal smooth muscle cells of DM group was significantly slower (13.33±4.27 vs 30.67±3.33, P <0.01), amplitude reduced significantly (15.17±3.71 vs 35.17±3.71, P <0.01), and the wave was irregular. The proximal duodenum in DM group appeared that the number of ICC was reduced, basement membrane was dissolved, organelle was damaged, endoplasmic reticulum was expanded, mitochondria was swollen, and even vacuolar degeneration; Close connections between ICC and the surrounding cells were destroyed.Conclusion: Diabetic mice have disorder of dudenum motility, which is closely related to abnormalities of ICC. Objective :To explore the effects of stem cell factor (SCF)on the Cajal interstitial cells of duodenum in diabetic mice.Methods: All Balb/c male mice were divided randomly into 3 groups: Control group, Diabetes Mellitus (DM) group and DM + SCF group. Diabetes mice models in DM group and DM + SCF group were established by intraperitoneal injection of streptozotocin(STZ, 150 mg/kg, ip) one time; then DM + SCF group was given SCF 0.20μg/(kg.d, ip). DM group and Control group were given phosphat buffer solution (pH = 7.40) at the same time everyday. After 6 weeks' intervention, all mice were given Indian ink by lavage for the small intestinal transit rate determination. The number of interstitial cells of cajal (ICC) in duodenal myenteric plexus was counted after immunohistochemistry staining. Expression of c-kit protein in duodenum was observed by Western blot.Results: After six weeks, body weight,small intestine transmission rate,the number of c-kit positive cells and the quantity of c-kit protein of DM+SCF group increased obviously compared with DM group respectively(P<0.01, P<0.001, P<0.05, P<0.05), still reduced obviously compared with Control group(P<0.01, P<0.005, P<0.05, P<0.05); Blood sugar in DM+SCF group was lower than that in DM group(P<0.01), still higher than that in Control group(P<0.05).Conclusion: Exogenous SCF maybe improve the duodenal motility in diabetic mice by inversing abnormal changes of ICC. Objective:To explore the changes of duodenal smooth muscle cells in diabetic mice and effects of stem cell factor (SCF) on smooth muscle cells.Methods: Mice were divided into three groups: Control group, Diabetes Mellitus (DM) group and DM + SCF group. DM models were established by intraperitoneal injection of streptozotocin(STZ, 150 mg/kg,ip) in Balb/c male mice. DM + SCF models were established by intraperitoneal injection of SCF0.20μg/(kg·d, ip) every day. Isodose phosphat buffer solution (pH = 7.40) was given in Control group and DM group. All the mice after 6 weeks, intervention, were given Indian ink by lavage for the small intestinal transit rate determination. Changes of duodenal smooth muscle cells were observed by HE stainning and transmission electron microscopy(TEM). Changes of slow waves of duodenal smooth muscle cells were recorded with intracellular recordings.Results:Small intestine transit rate of DM group was significantly reduced than that in Control group (44.05±5.48 vs 82.75±6.56, P < 0.01), but small intestine transit rate of DM + SCF group was significantly increased than that in DM group (75.89±3.61 vs 44.05±5.48, P < 0.01), but much lower than that in control group (75.89±3.61 vs 82.75±6.56, P < 0.05).Under light microscope, cytoplasmic of smooth muscle cell of DM group was obviously swollen, and that in DM+SCF group was alleviative significantly.Under TEM, mitochondrial was swollen, endoplasmic reticulum distended,even vacuolation was easily observed in DM group. Those in DM+SCF group were extenuated to some extent. Compared with the Control group, the frequency of slow waves of duodenal smooth muscle cells in DM group was more slower (13.33±4.27 vs 30.67±3.33, P < 0.01), and amplitude of duodenal smooth muscle cells in DM group was obviously lower (15.17±3.71vs35.17±3.71, P < 0.01), and the slow waves in DM group were irregular. The frequency and amplitude of slow waves of duodenal smooth muscle cells of DM + SCF group were obviously improved than that in DM group (26.50±1.87vs 13.33±4.27;27.50±2.26vs15.17±3.71, P < 0.01, respectively), but slower and lower than that in the Control group (26.50±1.87vs 30.67±3.33,P < 0.05; 27.50±2.26 vs35.17±3.71, P < 0.01).Conclusion:Exogenous SCF maybe inverse abnormal changes of smooth muscle cells and accordingly improve the disorder of duodenal motility in diabetic mices.
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