Determination And Comparison Of Trace Elements In Kidney Stone Patients Serum And Urine In Different Regions By ICP-MS

Background and ObjectivesA large number of epidemiological investigations, animal experiments and clinical study results indicate that there is a certain relationship between trace elements and Urolithiasis. it is paid more attention on the importance of trace elements in Urolithiasis in the fields of the basic research, clinical medicine, preventive medicine and its achievements with important scientific value and socioeconomic benefits. By establishing inductively coupled plasma mass spectrometry (induetively coupled Plasma mass speetrometry, ICP-MS) model to detect the content of serum and urine trace elements in kidney stone patients with calcium oxalate stone from different regions and the relationship between trace elements and regions.MethodsICP-MS was used to detect the content of serum and urine trace elements in three groups: 20 cases of healthy people, 21 cases of kidney stone patients with calcium oxalate stone(CaOXa) from Xiamen area and 23 cases from Longyan area.ResultsApplication of partial least squares discriminant analysis (PLS-DA) on kidney stone patients and healthy people serum and urine trace element analysis, healthy people'sample is dispersed, Xiamen and Longyan area cluster; There is a significant difference in three groups. The samples of kidney stone patients from Xiamen Longyan area and healthy people were clustered into three categories. The content of serum and urine trace elements in kidney stone patiens was significantly deviated from healthy people', as well as the groups from Xiamen and Longyan area. The metabolism pattern of trace elements has a significant differences in three groups. The content of magnesium, iron, thallium, zinc, manganese, chromium, selenium in kidney stone patients urine was lower than in healthy people (P<0.05), while calcium, aluminum, copper higher (P<0.05); The content of calcium, aluminum, copper, fluoride, cadmium in kidney stone patients serum was higher than healthy people(P<0.05), while magnesium, zinc lower (P<0.05); The content of urine magnesium, lithium and selenium in kidney stone patients from Longyan area was higher than from Xiamen area(P<0.05); The content of serum calcium and ferrum in kidney stone patients from Longyan area was higer than from Xiamen area (P<0.05).ConclusionsDue to significant difference on trace elements metabolism between kidney stone patients and healthy people, The ICP-MS can be used to detect the content of trace element by discriminant analysis.Longyan and Xiamen trace elements in in kidney stone patients with calcium oxalate stone had a significant difference, so as to provide the basis for the diagnosis and prevention in kidney stones patients from different regions.