MicroRNA, Inflammatory Pathway, NEIL2 Gene, And Esophageal Cancer

ObjectiveBy performing the epidemiological survey in Southern Fujian Province where exhibits a high incidence of esophageal cancer,to investigate the risk factors of esophageal cancer;to explore the relationship between MicroRNA polymorphism,inflammatory pathway,NEIL2 gene polymorphism,and esophageal cancer. From molecular level to discussion the pathogenesis and early diagnosis signs of esophageal cancer.The results will provide new insight into the etiology and thus the prevention of this carcinoma.Methords1,Using a hospital-based case-control study inculding 334 cases and 335 controls frequency-matched to the cases on age(±3 years) and gender were recuited,and surveyed by questionare.Using unconditional logistic regression model to evaluate risk factors,OR and 95%CI were computed.2,Designing molecular epidemiology study,peripheral blood of cases and controls were collected,and then their DNA were extracted.Inflammatory pathway related MicroRNA polymorphism were genotyped by MALDI-TOF-MS.Using Log_addivite,Dominate model,Recessive model three genetic models and unconditional logistic regression model to evaluate risks,OR and 95%CI were computed.Haplotype frequencies were computed by Haploview and PHASE software.3,Designing molecular epidemiology study,peripheral blood of cases and controls were collected,and then their DNA were extracted. First sequencing looking for common SNPs of NEIL2 , then the SNPs were genotyped by MALDI-TOF-MS.Using Log_addivite,Dominate model,Recessive model three genetic models and unconditional logistic regression model to evaluate risks,OR and 95%CI were computed.Haplotype frequencies were computed by Haploview and PHASE software.Results1,Risk factors of esophageal cancer included:Contact harmful substances OR=1.840(95% CI:1.202～2.186),smoked an average of 20 cigarettes a day OR=1.397(95% CI:1.130～1.726),eat mildew food OR=2.246(95% CI:1.258～4.011);protective factors of esophageal cancer included:dinner time≥10 min OR=0.741(95% CI:0.556～0.987),Cooking oil type OR=0.610(95% CI:0.529～0.703),Use the refrigerator OR=0.567(95% CI:0.376～0.853),Eat more fruit OR=0.603(95% CI:0.490～0.743)。2,Did not find meaningful polymorphic loci in Inflammatory pathway related MicroRNA polymorphism.But in stratified analysis rs829314 under Recessive model presents protective factor in more than 60 years of the population,OR is 0.39(95% CI:0.16-0.94);rs829314 also may presents protective factor in more than 60 years of the population,OR is 0.41(95% CI:0.17-1.00).3,Found two common SNPs of basal excision repair gene NEIL2,rs8191613and rs8191664.But Verified by case-control study,they had no relationship with esophageal cancer. But in stratified analysis rs8191613 under Dominate model presents risk factor in smoking population,OR is 1.60(95%CI:1.04-2.48). It indicated that esophageal cancer may exist gene-environment interaction.Conclusion1,Risk factors of esophageal cancer included:Contact harmful substances:chemical fertilizer and pesticide,smoked an average of 20 cigarettes a day,eat mildew food;protective factors of esophageal cancer included:dinner time≥10 min,Cooking oil is camellia oil and blend oil,Use the refrigerator,Eat more fruit。2,Inflammatory pathway related miRNA rs829314 in stratified analysis under Recessive model presents protective factor in more than 60 years of the population.3,Found two common SNPs of basal excision repair gene NEIL2,rs8191613and rs8191664.Rs8191613 under Dominate model presents risk factor in smoking population.It indicated that esophageal cancer may exist gene-environment interaction.