| In this paper, anti-tumor and antioxidation activity constituent of fruiting body of Phellinus vaninii were studied.On anti-tumor activity, inhibitory effects of the petroleum ether extraction, chloroform extraction, acetone extraction and water extraction on the growth of H22 tumor-bearing mice were detected in vivo. The result showed that the petroleum ether extraction, chloroform extraction of high dosage group (100mg/kg) and acetone extraction of high dosage group (100mg/kg) all have manifest antitumor effectivity, and they could regulate the immune function of tumor-bearing mice effectively and prolong survival in mice significantly. The petroleum ether extraction of high dosage group (100mg/kg) has the best anti-tumor activity and the rate has reached 48.62%, and closed the rate of CTX group (56.09%). The spleen index of petroleum ether extraction and acetone extraction of high dosage group (100mg/kg) were significantly higher than them of the control, CTX groups and normal group(P<0.01). The thymus index of petroleum ether extraction, chloroform extraction and acetone extraction were significantly higher than them of the control and CTX groups, closing to the normal group(P<0.01). The contents of IL-2 of the petroleum ether extraction, chloroform extraction of high dosage group (100mg/kg) and acetone extraction were significantly higher than them of the CTX groups and control group(P<0.01).At the same time, the subjected drugs could prolong the survival period of H22 mice, in which the petroleum ether extraction, chloroform extraction of low dosage group (50mg/kg) and acetone extraction of low dosage group (50mg/kg) and the water extraction of low dosage group (500mg/kg) can significantly improve the survival rate of H22 mice, and the survival rate have separately reached 52.58%,52.58%,53.09%,47.42%and 54.64%. From this description, petroleum ether extraction, chloroform extraction and acetone extraction of fruiting body of Phellinus vaninii can effectively inhibit tumor growth, and can improve the body's immune system..In addition, seven compounds were separated from the petroleum ether extraction, chloroform extraction and acetone extraction of fruiting body of Phellinus vaninii by silica-gel column chromatography and C18 column chromatography and identified by MS,1H-NMR,13C-NMR and DEPT. The seven compounds are 4,6,8(14),22(23)-tetraen-3-one-ergostane, sakuranetin,7-methoxy-dihydrokaempferol, 4-(3,4-dihydro-xyph-enyl)-3-buten-2-one, dihydrokaempferol, hispolon and 3',4', 5,-trihydroxy-7-methoxy-dihydro-flavonol. And all the compounds were isolated from the fruiting body of Phellinus vaninii for the first time. Antitumor activity of sakuranetin,7-methoxy-dihydrokaempferol,4-(3,4-dihydro-xyphenyl)-3-buten-2-one, dihydrokaempferol and hispolon from the fruiting body of Phellinus vaninii was studied by MTT method in vitro.The results indicated that 4-(3,4-dihydro-xyphenyl)-3-buten-2-one and hispolon has both a certain effect on human liver cancer cells SMMC-7721 and human breast cancer cells MCF-7 in vitro. Their inhibitory ratio on SMMC-7721 cells was 34.83% and 48.09% and on MCF-7 cells was 71.09% and 74.57% at 100μg/mL, respectively.The IC50 values were 69.48μg/mL,61.57μg/mL,30.22μg/mL and 24.68μg/mL,respectively. Therefore, based on the comprehensive analysis, we can identify that 4-(3,4-dihydroxy-phenyl)-3-butene-2-one and hispolon have good inhibitory effect on proliferation of SMMC-7721 cells and MCF-7 cells, they are anti-tumor activity constituent of fruiting body of Phellinus vaninii.In the antioxidation experiment, in vitro 1,1-diphenyl-2-picrylhydrazyl radical (·DPPH), superoxide anion radical (·O2-) and hydroxyl radical (·OH) test systems were established to study the free radical scavenging activities of sakuranetin,7-methoxy-dihydrokaempferol,4-(3,4- dihydro-xyphenyl)-3-buten-2-one, dihydrokaempferol and hispolon. The results showed that·DPPH radical scavenging ratio of 4-(3,4-dihydroxy-phenyl)-3-butene-2-one and hispolon was 92.02% and 92.61%,·OH radical scavenging ratio was 90.48% and 95.24%, while·O2- radical scavenging ratio was 69.62% and 76.82% at 100μg/mL, respectively. The·O2- radical scavenging ratio of dihydrokaempferol was 39.02%. Nevertheless, all the free radical scavenging ratio of sakuranetin and 7-methoxy-dihydrokaempferol was less than 30%. Therefore, 4-(3,4-dihydroxy-phenyl)-3-butene-2-one and hispolon are antioxidation activity constituent of fruiting body of Phellinus vanini.
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