Clinical Research On The Effect Of Xiaomengling On TNF-α And JAK2/STAT5 Pathway In Retina Of Diabetes Mellitus Rats

ObjectivesTo evaluate the clinical effect of Xiaomengling on the DR patients; To research the effect of Xiaomengling on TNF-αand JAK2/STAT5 pathway in retina of Diabetes Mellitus rats MethodsFifty male Sprague-Dawley rats were randomly divided into normal control group, model control group and three treatment groups. The rats in normal control group give the food and water normally, the model control group only give the saline(NS) instead of the drug after injected STZ, and the rats in treatment group receive the different dose of xiaomengling treatment. After 5 months, observed the structure of retina, detect the protein expression of TNF-α, JAK2 and STAT5 by western-bloting.Observe the patients who used xiaomengling treatment before and after, compare the patients' vision, the hemorrhage of retina and microaneurysm. ResultCompare the effect of normal control group, model control group and treatment groups, weight and blood sugar of the rats, the discrepancy is obvious (P<0.05); the discrepancy in the kinds of treatment groups is also obvious (P<0.05); the protein expression levels of TNFα, JAK2 and STAT5 were declined in treatment groups, the high dose treatment group was the lowest, the middle dose treatment group was next, following were the low dose treatment group and normal control group, the protein level of TNF-α, JAK2 and STAT5 in model control group was the highest. The retinal structure of model control group showed retinal vessel is not shape and edema of retinal inner limiting membrane and irregular cells, these changes was most serious in model control group, the treatment group was not serious, the normal control group is normal. The transmission electron microscope showed there were disorders in the retinal neure, gap enlarged between membranes disc and cell decreased at exterior and interior nuclear layer.Compare the effect before treatment and after, analyst the vision, the hemorrhage of retina, microaneurysm, the effect after treatment is better than before (P<0.05). ConclusionTNF-α, JAK2/STAT5 play an important role in the pathologic process of DR. Xiaomengling can declined the the protein level of TNF-α, JAK2 and STAT5 in order to delay the process of DR.